Structural basis of a nucleosome containing histone H2A.B/H2A.Bbd that transiently associates with reorganized chromatin

Yasuhiro Arimura, Hiroshi Kimura, Takashi Oda, Koichi Sato, Akihisa Osakabe, Hiroaki Tachiwana, Yuko Sato, Yasuha Kinugasa, Tsuyoshi Ikura, Masaaki Sugiyama, Mamoru Sato, Hitoshi Kurumizaka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Human histone H2A.B (formerly H2A.Bbd), a non-allelic H2A variant, exchanges rapidly as compared to canonical H2A, and preferentially associates with actively transcribed genes. We found that H2A.B transiently accumulated at DNA replication and repair foci in living cells. To explore the biochemical function of H2A.B, we performed nucleosome reconstitution analyses using various lengths of DNA. Two types of H2A.B nucleosomes, octasome and hexasome, were formed with 116, 124, or 130 base pairs (bp) of DNA, and only the octasome was formed with 136 or 146 bp DNA. In contrast, only hexasome formation was observed by canonical H2A with 116 or 124 bp DNA. A small-angle X-ray scattering analysis revealed that the H2A.B octasome is more extended, due to the flexible detachment of the DNA regions at the entry/exit sites from the histone surface. These results suggested that H2A.B rapidly and transiently forms nucleosomes with short DNA segments during chromatin reorganization.

Original languageEnglish
Article number3510
JournalScientific Reports
Volume3
DOIs
Publication statusPublished - 2013 Dec 16
Externally publishedYes

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Nucleosomes
Histones
Chromatin
DNA
Base Pairing
DNA Replication
DNA Repair
X-Rays
Genes

ASJC Scopus subject areas

  • General

Cite this

Arimura, Y., Kimura, H., Oda, T., Sato, K., Osakabe, A., Tachiwana, H., ... Kurumizaka, H. (2013). Structural basis of a nucleosome containing histone H2A.B/H2A.Bbd that transiently associates with reorganized chromatin. Scientific Reports, 3, [3510]. https://doi.org/10.1038/srep03510

Structural basis of a nucleosome containing histone H2A.B/H2A.Bbd that transiently associates with reorganized chromatin. / Arimura, Yasuhiro; Kimura, Hiroshi; Oda, Takashi; Sato, Koichi; Osakabe, Akihisa; Tachiwana, Hiroaki; Sato, Yuko; Kinugasa, Yasuha; Ikura, Tsuyoshi; Sugiyama, Masaaki; Sato, Mamoru; Kurumizaka, Hitoshi.

In: Scientific Reports, Vol. 3, 3510, 16.12.2013.

Research output: Contribution to journalArticle

Arimura, Y, Kimura, H, Oda, T, Sato, K, Osakabe, A, Tachiwana, H, Sato, Y, Kinugasa, Y, Ikura, T, Sugiyama, M, Sato, M & Kurumizaka, H 2013, 'Structural basis of a nucleosome containing histone H2A.B/H2A.Bbd that transiently associates with reorganized chromatin', Scientific Reports, vol. 3, 3510. https://doi.org/10.1038/srep03510
Arimura, Yasuhiro ; Kimura, Hiroshi ; Oda, Takashi ; Sato, Koichi ; Osakabe, Akihisa ; Tachiwana, Hiroaki ; Sato, Yuko ; Kinugasa, Yasuha ; Ikura, Tsuyoshi ; Sugiyama, Masaaki ; Sato, Mamoru ; Kurumizaka, Hitoshi. / Structural basis of a nucleosome containing histone H2A.B/H2A.Bbd that transiently associates with reorganized chromatin. In: Scientific Reports. 2013 ; Vol. 3.
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