Structural basis of instability of the nucleosome containing a testis-specific histone variant, human H3T

Hiroaki Tachiwana, Wataru Kagawa, Akihisa Osakabe, Koichiro Kawaguchi, Tatsuya Shiga, Yoko Hayashi-Takanaka, Hiroshi Kimura, Hitoshi Kurumizaka

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    126 Citations (Scopus)

    Abstract

    A histone H3 variant, H3T, is highly expressed in the testis, suggesting that it may play an important role in the chromatin reorganization required for meiosis and/or spermatogenesis. In the present study, we found that the nucleosome containing human H3T is significantly unstable both in vitro and in vivo, as compared to the conventional nucleosome containing H3.1. The crystal structure of the H3T nucleosome revealed structural differences in the H3T regions on both ends of the central α2 helix, as compared to those of H3.1. The H3T-specific residues (Met71 and Val111) are the source of the structural differences observed between H3T and H3.1. A mutational analysis revealed that these residues are responsible for the reduced stability of the H3T-containing nucleosome. These physical and structural properties of the H3T-containing nucleosome may provide the basis of chromatin reorganization during spermatogenesis.

    Original languageEnglish
    Pages (from-to)10454-10459
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume107
    Issue number23
    DOIs
    Publication statusPublished - 2010 Jun 8

    ASJC Scopus subject areas

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    Tachiwana, H., Kagawa, W., Osakabe, A., Kawaguchi, K., Shiga, T., Hayashi-Takanaka, Y., Kimura, H., & Kurumizaka, H. (2010). Structural basis of instability of the nucleosome containing a testis-specific histone variant, human H3T. Proceedings of the National Academy of Sciences of the United States of America, 107(23), 10454-10459. https://doi.org/10.1073/pnas.1003064107