Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria

Tsutomu Suzuki, Maki Terasaki, Chie Takemoto-Hori, Takao Hanada, Takuya Ueda, Akira Wada, Kimitsuna Watanabe

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Abstract

The mammalian mitochondrial ribosome (mitoribosome) is a highly protein-rich particle in which almost half of the rRNA contained in the bacterial ribosome is replaced with proteins. It is known that mitochondrial translation factors can function on both mitochondrial and Escherichia coli ribosomes, indicating that protein components in the mitoribosome compensate the reduced rRNA chain to make a bacteria-type ribosome. To elucidate the molecular basis of this compensation, we analyzed bovine mitoribosomal large subunit proteins; 31 proteins were identified including 15 newly identified proteins with their cDNA sequences from human and mouse. The results showed that the proteins with binding sites on rRNA shortened or lost in the mitoribosome were enlarged when compared with the E. coli counterparts; this suggests the structural compensation of the rRNA deficit by the enlarged proteins in the mitoribosome.

Original languageEnglish
Pages (from-to)21724-21736
Number of pages13
JournalJournal of Biological Chemistry
Volume276
Issue number24
DOIs
Publication statusPublished - 2001 Jun 15

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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