Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1

Yasuyuki Kurihara, Takashi Nagata, Takao Imai, Ado Hiwatashi, Masataka Horiuchi, Shinichi Sakakibara, Masato Katahira, Hideyuki Okano, Seiichi Uesugi

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mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
Issue number1
Publication statusPublished - 1997 Feb 20
Externally publishedYes



  • α-helix
  • β-sheet
  • Circular dichroism
  • Elav
  • Musashi
  • Northwestern blotting
  • Secondary structure
  • U1 snRNP A

ASJC Scopus subject areas

  • Genetics

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