Structural property of DNA that migrates faster in gel electrophoresis, as deduced by CD spectroscopy

Akimasa Matsugami, Keiko Tani, Kiyoshi Ouhashi, Seiichi Uesugi, Mitsunori Morita, Takashi Ohyama, Masato Katahira

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Bent DNAs are known to migrate slower than ordinary DNA in non-denaturing polyacrylamide gel electrophoresis. In contrast, several satellite DNAs have been shown to migrate fast. The structural property that causes the fast migration, however, is not clarified so far on molecular basis. We have investigated the structural property of a satellite DNA, which contains consecutive purine sequences and migrates faster in gel, by CD spectroscopy. Partial formation of an A-form-like structure has been suggested. Reduction in DNA length due to the formation of the A-form-like structure may be responsible for the fast migration. The pronounced rigidity of DNA may also contribute to the behavior.

Original languageEnglish
Pages (from-to)417-425
Number of pages9
JournalNucleosides, Nucleotides and Nucleic Acids
Volume25
Issue number4-6
DOIs
Publication statusPublished - 2006 Jun 1
Externally publishedYes

Fingerprint

Electrophoresis
Structural properties
Spectrum Analysis
Gels
Spectroscopy
Satellite DNA
DNA
Native Polyacrylamide Gel Electrophoresis
Rigidity

Keywords

  • A-Form
  • Bent DNA
  • CD
  • Satellite DNA

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Structural property of DNA that migrates faster in gel electrophoresis, as deduced by CD spectroscopy. / Matsugami, Akimasa; Tani, Keiko; Ouhashi, Kiyoshi; Uesugi, Seiichi; Morita, Mitsunori; Ohyama, Takashi; Katahira, Masato.

In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 25, No. 4-6, 01.06.2006, p. 417-425.

Research output: Contribution to journalArticle

Matsugami, Akimasa ; Tani, Keiko ; Ouhashi, Kiyoshi ; Uesugi, Seiichi ; Morita, Mitsunori ; Ohyama, Takashi ; Katahira, Masato. / Structural property of DNA that migrates faster in gel electrophoresis, as deduced by CD spectroscopy. In: Nucleosides, Nucleotides and Nucleic Acids. 2006 ; Vol. 25, No. 4-6. pp. 417-425.
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