Structure and Expression of Novel Protein-Tyrosine Kinases, EMB and EMT, in Hematopoietic Cells

Nobuo Yamada, Yuko Kawakami, Hitoshi Kimura, Hiromi Fukamachi, Gottfried Baier, Amnon Altman, Takashi Kato, Yoshimasa Inagaki, Toshiaki Kawakami

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Two novel tyrosine kinase cDNAs were obtained from murine mast cells. These kinases, Emb and Emt, constitute a novel tyrosine kinase subfamily which may also include Tec, a kinase preferentially expressed in liver, and Dsrc28, a fruit fly kinase. Both lack hydrophobic stretches characteristic of the transmembrane domains found in growth factor receptor tyrosine kinases and carboxyl-terminal, negative regulatory tyrosine residue found in Src family kinases. In addition to the Src homology region 2 (SH2) and SH3 domains characteristic of the Src family kinases and other signaling molecules, Emb and Emt share a similar amino-terminal domain comprised mainly of two repeat segments. The emb 2.7-kb transcript was expressed in mast cells, myeloid cells and B lymphocytes while the emt 4.6-kb mRNA in mast cells, myeloid cells and T lymphocytes. The evidence for in vitro tyrosine kinase activity of Emb and Emt proteins is also provided.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume192
Issue number1
DOIs
Publication statusPublished - 1993 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Structure and Expression of Novel Protein-Tyrosine Kinases, EMB and EMT, in Hematopoietic Cells'. Together they form a unique fingerprint.

  • Cite this

    Yamada, N., Kawakami, Y., Kimura, H., Fukamachi, H., Baier, G., Altman, A., Kato, T., Inagaki, Y., & Kawakami, T. (1993). Structure and Expression of Novel Protein-Tyrosine Kinases, EMB and EMT, in Hematopoietic Cells. Biochemical and Biophysical Research Communications, 192(1), 231-240. https://doi.org/10.1006/bbrc.1993.1404