Structure and Expression of Novel Protein-Tyrosine Kinases, EMB and EMT, in Hematopoietic Cells

N. Yamada, Y. Kawakami, H. Kimura, H. Fukamachi, G. Baier, A. Altman, Takashi Kato, Y. Inagaki, T. Kawakami

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Two novel tyrosine kinase cDNAs were obtained from murine mast cells. These kinases, Emb and Emt, constitute a novel tyrosine kinase subfamily which may also include Tec, a kinase preferentially expressed in liver, and Dsrc28, a fruit fly kinase. Both lack hydrophobic stretches characteristic of the transmembrane domains found in growth factor receptor tyrosine kinases and carboxyl-terminal, negative regulatory tyrosine residue found in Src family kinases. In addition to the Src homology region 2 (SH2) and SH3 domains characteristic of the Src family kinases and other signaling molecules, Emb and Emt share a similar amino-terminal domain comprised mainly of two repeat segments. The emb 2.7-kb transcript was expressed in mast cells, myeloid cells and B lymphocytes while the emt 4.6-kb mRNA in mast cells, myeloid cells and T lymphocytes. The evidence for in vitro tyrosine kinase activity of Emb and Emt proteins is also provided.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume192
Issue number1
DOIs
Publication statusPublished - 1993 Apr 15
Externally publishedYes

Fingerprint

Mast Cells
Protein-Tyrosine Kinases
src Homology Domains
src-Family Kinases
Myeloid Cells
Phosphotransferases
Growth Factor Receptors
Diptera
T-cells
Tyrosine
Lymphocytes
Fruit
B-Lymphocytes
Complementary DNA
Fruits
Liver
T-Lymphocytes
Messenger RNA
Molecules
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

Structure and Expression of Novel Protein-Tyrosine Kinases, EMB and EMT, in Hematopoietic Cells. / Yamada, N.; Kawakami, Y.; Kimura, H.; Fukamachi, H.; Baier, G.; Altman, A.; Kato, Takashi; Inagaki, Y.; Kawakami, T.

In: Biochemical and Biophysical Research Communications, Vol. 192, No. 1, 15.04.1993, p. 231-240.

Research output: Contribution to journalArticle

Yamada, N, Kawakami, Y, Kimura, H, Fukamachi, H, Baier, G, Altman, A, Kato, T, Inagaki, Y & Kawakami, T 1993, 'Structure and Expression of Novel Protein-Tyrosine Kinases, EMB and EMT, in Hematopoietic Cells', Biochemical and Biophysical Research Communications, vol. 192, no. 1, pp. 231-240. https://doi.org/10.1006/bbrc.1993.1404
Yamada, N. ; Kawakami, Y. ; Kimura, H. ; Fukamachi, H. ; Baier, G. ; Altman, A. ; Kato, Takashi ; Inagaki, Y. ; Kawakami, T. / Structure and Expression of Novel Protein-Tyrosine Kinases, EMB and EMT, in Hematopoietic Cells. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 192, No. 1. pp. 231-240.
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