Structure and function of a family 10 β-xylanase chimera of Streptomyces olivaceoviridis E-86 FXYN and Cellulomonas fimi cex

Satoshi Kaneko, Hitomi Ichinose, Zui Fujimoto, Atsushi Kuno, Kei Yura, Mitiko Go, Hiroshi Mizuno, Isao Kusakabe, Hideyuki Kobayashi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The catalytic domain of xylanases belonging to glycoside hydrolase family 10 (GH10) can be divided into 22 modules (M1 to M22; Sato, Y., Niimura, Y., Yura, K., and Go, M. (1999) Gene (Amst.) 238, 93-101). Inspection of the crystal structure of a GH10 xylanase from Streptomyces olivaceoviridis E-86 (SoXyn10A) revealed that the catalytic domain of GH10 xylanases can be dissected into two parts, an N-terminal larger region and C-terminal smaller region, by the substrate binding cleft, corresponding to the module border between M14 and M15. It has been suggested that the topology of the substrate binding clefts of GH10 xylanases are not conserved (Charnock, S. J., Spurway, T. D., Xie, H., Beylot, M. H., Virden, R., Warren, R. A. J., Hazlewood, G. P., and Gilbert, H. J. (1998) J. Biol. Chem. 273, 32187-32199). To facilitate a greater understanding of the structure-function relationship of the substrate binding cleft of GH10 xylanases, a chimeric xylanase between SoXyn10A and Xyn10A from Cellulomonas fimi (CgXyn10A) was constructed, and the topology of the hybrid substrate binding cleft established. At the three-dimensional level, SoXyn10A and CfXyn10A appear to possess 5 subsites, with the amino acid residues comprising subsites -3 to +1 being well conserved, although the +2 subsites are quite different. Biochemical analyses of the chimeric enzyme along with SoXyn10A and CfXyn10A indicated that differences in the structure of subsite +2 influence bond cleavage frequencies and the catalytic efficiency of xylooligosaccharide hydrolysis. The hybrid enzyme constructed in this study displays fascinating biochemistry, with an interesting combination of properties from the parent enzymes, resulting in a low production of xylose.

Original languageEnglish
Pages (from-to)26619-26626
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number25
DOIs
Publication statusPublished - 2004 Jun 18
Externally publishedYes

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Cellulomonas
Glycoside Hydrolases
Streptomyces
Substrates
Catalytic Domain
Enzymes
Topology
Biochemistry
Xylose
Hydrolysis
Genes
Crystal structure
Inspection
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Structure and function of a family 10 β-xylanase chimera of Streptomyces olivaceoviridis E-86 FXYN and Cellulomonas fimi cex. / Kaneko, Satoshi; Ichinose, Hitomi; Fujimoto, Zui; Kuno, Atsushi; Yura, Kei; Go, Mitiko; Mizuno, Hiroshi; Kusakabe, Isao; Kobayashi, Hideyuki.

In: Journal of Biological Chemistry, Vol. 279, No. 25, 18.06.2004, p. 26619-26626.

Research output: Contribution to journalArticle

Kaneko, S, Ichinose, H, Fujimoto, Z, Kuno, A, Yura, K, Go, M, Mizuno, H, Kusakabe, I & Kobayashi, H 2004, 'Structure and function of a family 10 β-xylanase chimera of Streptomyces olivaceoviridis E-86 FXYN and Cellulomonas fimi cex', Journal of Biological Chemistry, vol. 279, no. 25, pp. 26619-26626. https://doi.org/10.1074/jbc.M308899200
Kaneko, Satoshi ; Ichinose, Hitomi ; Fujimoto, Zui ; Kuno, Atsushi ; Yura, Kei ; Go, Mitiko ; Mizuno, Hiroshi ; Kusakabe, Isao ; Kobayashi, Hideyuki. / Structure and function of a family 10 β-xylanase chimera of Streptomyces olivaceoviridis E-86 FXYN and Cellulomonas fimi cex. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 25. pp. 26619-26626.
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abstract = "The catalytic domain of xylanases belonging to glycoside hydrolase family 10 (GH10) can be divided into 22 modules (M1 to M22; Sato, Y., Niimura, Y., Yura, K., and Go, M. (1999) Gene (Amst.) 238, 93-101). Inspection of the crystal structure of a GH10 xylanase from Streptomyces olivaceoviridis E-86 (SoXyn10A) revealed that the catalytic domain of GH10 xylanases can be dissected into two parts, an N-terminal larger region and C-terminal smaller region, by the substrate binding cleft, corresponding to the module border between M14 and M15. It has been suggested that the topology of the substrate binding clefts of GH10 xylanases are not conserved (Charnock, S. J., Spurway, T. D., Xie, H., Beylot, M. H., Virden, R., Warren, R. A. J., Hazlewood, G. P., and Gilbert, H. J. (1998) J. Biol. Chem. 273, 32187-32199). To facilitate a greater understanding of the structure-function relationship of the substrate binding cleft of GH10 xylanases, a chimeric xylanase between SoXyn10A and Xyn10A from Cellulomonas fimi (CgXyn10A) was constructed, and the topology of the hybrid substrate binding cleft established. At the three-dimensional level, SoXyn10A and CfXyn10A appear to possess 5 subsites, with the amino acid residues comprising subsites -3 to +1 being well conserved, although the +2 subsites are quite different. Biochemical analyses of the chimeric enzyme along with SoXyn10A and CfXyn10A indicated that differences in the structure of subsite +2 influence bond cleavage frequencies and the catalytic efficiency of xylooligosaccharide hydrolysis. The hybrid enzyme constructed in this study displays fascinating biochemistry, with an interesting combination of properties from the parent enzymes, resulting in a low production of xylose.",
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AU - Fujimoto, Zui

AU - Kuno, Atsushi

AU - Yura, Kei

AU - Go, Mitiko

AU - Mizuno, Hiroshi

AU - Kusakabe, Isao

AU - Kobayashi, Hideyuki

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