Structure and function of human histone H3.Y nucleosome

Tomoya Kujirai, Naoki Horikoshi, Koichi Sato, Kazumitsu Maehara, Shinichi Machida, Akihisa Osakabe, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Histone H3.Y is a primate-specific, distant H3 variant. It is evolutionarily derived from H3.3, and may function in transcription regulation. However, the mechanism by which H3.Y regulates transcription has not been elucidated. In the present study, we determined the crystal structure of the H3.Y nucleosome, and found that many H3.Y-specific residues are located on the entry/exit sites of the nucleosome. Biochemical analyses revealed that the DNA ends of the H3.Y nucleosome were more flexible than those of the H3.3 nucleosome, although the H3.Y nucleosome was stable in vitro and in vivo. Interestingly, the linker histone H1, which compacts nucleosomal DNA, appears to bind to the H3.Y nucleosome less efficiently, as compared to the H3.3 nucleosome. These characteristics of the H3.Y nucleosome are also conserved in the H3.Y/H3.3 heterotypic nucleosome, which may be the predominant form in cells. In human cells, H3.Y preferentially accumulated around transcription start sites (TSSs). Taken together, H3.Y-containing nucleosomes around transcription start sites may form relaxed chromatin that allows transcription factor access, to regulate the transcription status of specific genes.

Original languageEnglish
Pages (from-to)6127-6141
Number of pages15
JournalNucleic Acids Research
Volume44
Issue number13
DOIs
Publication statusPublished - 2016 Jul 27

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Nucleosomes
Histones
Transcription Initiation Site
DNA
Primates
Chromatin
Transcription Factors

ASJC Scopus subject areas

  • Genetics

Cite this

Kujirai, T., Horikoshi, N., Sato, K., Maehara, K., Machida, S., Osakabe, A., ... Kurumizaka, H. (2016). Structure and function of human histone H3.Y nucleosome. Nucleic Acids Research, 44(13), 6127-6141. https://doi.org/10.1093/nar/gkw202

Structure and function of human histone H3.Y nucleosome. / Kujirai, Tomoya; Horikoshi, Naoki; Sato, Koichi; Maehara, Kazumitsu; Machida, Shinichi; Osakabe, Akihisa; Kimura, Hiroshi; Ohkawa, Yasuyuki; Kurumizaka, Hitoshi.

In: Nucleic Acids Research, Vol. 44, No. 13, 27.07.2016, p. 6127-6141.

Research output: Contribution to journalArticle

Kujirai, T, Horikoshi, N, Sato, K, Maehara, K, Machida, S, Osakabe, A, Kimura, H, Ohkawa, Y & Kurumizaka, H 2016, 'Structure and function of human histone H3.Y nucleosome', Nucleic Acids Research, vol. 44, no. 13, pp. 6127-6141. https://doi.org/10.1093/nar/gkw202
Kujirai T, Horikoshi N, Sato K, Maehara K, Machida S, Osakabe A et al. Structure and function of human histone H3.Y nucleosome. Nucleic Acids Research. 2016 Jul 27;44(13):6127-6141. https://doi.org/10.1093/nar/gkw202
Kujirai, Tomoya ; Horikoshi, Naoki ; Sato, Koichi ; Maehara, Kazumitsu ; Machida, Shinichi ; Osakabe, Akihisa ; Kimura, Hiroshi ; Ohkawa, Yasuyuki ; Kurumizaka, Hitoshi. / Structure and function of human histone H3.Y nucleosome. In: Nucleic Acids Research. 2016 ; Vol. 44, No. 13. pp. 6127-6141.
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