Structure and function of small heat shock proteins from the magnetotactic bacterium Magnetospirillum magneticum AMB-1

Tetsuya Abe, Shinpei Ito, Naoya Nishi, Yoshihiro Tsukada, Takuo Yasunaga, Atsushi Arakaki, Tadashi Matsunaga, Masafumi Yohda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this study, we expressed, purified and characterized small heat shock proteins (sHsp) from a magnetotactic bacterium, Magnetospirillum magneticm AMB-1, Hsp17.5, Hsp17.8 and Hsp18.3. They all showed molecular chaperone activity to protect citrate synthase from thermal aggregation at 45°C. The oligomeric states were examined by size exclusion chromatography and electron microscopy. Hsp17.8 exists as a spherical oligomer like other sHsps, and the oligomer reversibly dissociates to small conformers, probably dimers, at elevated temperatures. On the contrary, Hsp17.5 and Hsp18.3 take large filamentous conformations. A similar structure was observed in an sHsp of Sulfolobus tokodaii, StHsp19.7, but it did not exhibit molecular chaperone activity. Hsp17.5 and Hsp18.3 changed their oligomeric states according to the elevation of temperature. Among them, Hsp17.5 exhibited reversible dissociation. This is the first report that the filamentous sHsp exhibit molecular chaperone activity with the change of oligomeric states.

Original languageEnglish
Pages (from-to)698-704
Number of pages7
JournalKobunshi Ronbunshu
Volume67
Issue number12
DOIs
Publication statusPublished - 2010 Dec
Externally publishedYes

Keywords

  • Assembly and dissociation
  • Homo-Oligomer
  • Magnetospirillum magneticum AMB-1
  • Small heat shock protein

ASJC Scopus subject areas

  • Polymers and Plastics
  • Environmental Science(all)
  • Materials Science (miscellaneous)
  • Chemical Engineering (miscellaneous)

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  • Cite this

    Abe, T., Ito, S., Nishi, N., Tsukada, Y., Yasunaga, T., Arakaki, A., Matsunaga, T., & Yohda, M. (2010). Structure and function of small heat shock proteins from the magnetotactic bacterium Magnetospirillum magneticum AMB-1. Kobunshi Ronbunshu, 67(12), 698-704. https://doi.org/10.1295/koron.67.698