Structure of mitochondrial tRNA.

T. Yokogawa, Y. Watanabe, Y. Yotsumoto, Y. Kumazawa, Takuya Ueda, I. Hirao, K. Miura, K. Watanabe

Research output: Contribution to journalArticle

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Abstract

Some mitochondrial (mt) tRNAs are presumed to have unusual secondary structures on the basis of DNA sequence analyses. However there are few tRNAs whose structures have been investigated at RNA level. Thus we isolated some mt tRNAs to investigated their structures and also synthesized mt tRNA by run-off transcription. In these experiments, following results have been obtained. 1) Bovine mt tRNA(Ser)(AGY) needs the interaction between the T-loop and D-arm for its aminoacylation activity. 2) The secondary structure of bovine mt tRNA(Ser)(UCN) is difficult from that predicted previously from its gene. 3) It has been confirmed by the hybridization using oligonucleotide probes that some Ascaris suum mt tRNAs possessing the unusual T-arms actually exist in its mitochondria.

Original languageEnglish
Pages (from-to)175-176
Number of pages2
JournalNucleic acids symposium series
Issue number25
Publication statusPublished - 1991 Jan 1
Externally publishedYes

Fingerprint

Transfer RNA
RNA, Transfer, Ser
Ascaris suum
Aminoacylation
Oligonucleotide Probes
DNA Sequence Analysis
Mitochondria
RNA
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Yokogawa, T., Watanabe, Y., Yotsumoto, Y., Kumazawa, Y., Ueda, T., Hirao, I., ... Watanabe, K. (1991). Structure of mitochondrial tRNA. Nucleic acids symposium series, (25), 175-176.

Structure of mitochondrial tRNA. / Yokogawa, T.; Watanabe, Y.; Yotsumoto, Y.; Kumazawa, Y.; Ueda, Takuya; Hirao, I.; Miura, K.; Watanabe, K.

In: Nucleic acids symposium series, No. 25, 01.01.1991, p. 175-176.

Research output: Contribution to journalArticle

Yokogawa, T, Watanabe, Y, Yotsumoto, Y, Kumazawa, Y, Ueda, T, Hirao, I, Miura, K & Watanabe, K 1991, 'Structure of mitochondrial tRNA.', Nucleic acids symposium series, no. 25, pp. 175-176.
Yokogawa T, Watanabe Y, Yotsumoto Y, Kumazawa Y, Ueda T, Hirao I et al. Structure of mitochondrial tRNA. Nucleic acids symposium series. 1991 Jan 1;(25):175-176.
Yokogawa, T. ; Watanabe, Y. ; Yotsumoto, Y. ; Kumazawa, Y. ; Ueda, Takuya ; Hirao, I. ; Miura, K. ; Watanabe, K. / Structure of mitochondrial tRNA. In: Nucleic acids symposium series. 1991 ; No. 25. pp. 175-176.
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