Structure of the human CCG1 gene

relationship between the exons/introns and functional domain/modules of the protein

Nakashima Torahiko, Sekiguchi Takeshi, Sunamoto Hidetoshi, Kei Yura, Tomoda Shirou, Go Mitiko, Kere Juha, Schlessinger David, Nishimoto Takeharu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The human CCG1 gene, encoding CCGl/TAFII250/p250, was isolated by complementing tsBN462, a mutant BHK21 cell line that shows cell-cycle arrest at high temperature. Using the cDNA as a probe, the locations of exon-intron junctions were determined in the genomic DNA. Thirty-eight exons ranging from 68 to 219 bp in size were found. All the exonintron junctions followed the GT-AG rule. Using a newly developed method, we performed a module analysis of the CCG1 protein. The functional domain previously predicted in CCG1 was further confirmed to be encoded in a single predicted module that is the minimal functional unit in the protein. The boundaries of the predicted modules show a close correlation to the intron/exon junction of CCG1. The entire gene, at least 110kb long, has been recovered in a YAC, which provides a route to the further study of module function.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalGene
Volume141
Issue number2
DOIs
Publication statusPublished - 1994 Apr 20
Externally publishedYes

Fingerprint

Introns
Exons
Genes
Cell Cycle Checkpoints
Proteins
Complementary DNA
Cell Line
Temperature
DNA
Protein Domains

Keywords

  • Cell cycle
  • TAF250
  • tsBN462 cells

ASJC Scopus subject areas

  • Genetics

Cite this

Structure of the human CCG1 gene : relationship between the exons/introns and functional domain/modules of the protein. / Torahiko, Nakashima; Takeshi, Sekiguchi; Hidetoshi, Sunamoto; Yura, Kei; Shirou, Tomoda; Mitiko, Go; Juha, Kere; David, Schlessinger; Takeharu, Nishimoto.

In: Gene, Vol. 141, No. 2, 20.04.1994, p. 193-200.

Research output: Contribution to journalArticle

Torahiko, N, Takeshi, S, Hidetoshi, S, Yura, K, Shirou, T, Mitiko, G, Juha, K, David, S & Takeharu, N 1994, 'Structure of the human CCG1 gene: relationship between the exons/introns and functional domain/modules of the protein', Gene, vol. 141, no. 2, pp. 193-200. https://doi.org/10.1016/0378-1119(94)90570-3
Torahiko, Nakashima ; Takeshi, Sekiguchi ; Hidetoshi, Sunamoto ; Yura, Kei ; Shirou, Tomoda ; Mitiko, Go ; Juha, Kere ; David, Schlessinger ; Takeharu, Nishimoto. / Structure of the human CCG1 gene : relationship between the exons/introns and functional domain/modules of the protein. In: Gene. 1994 ; Vol. 141, No. 2. pp. 193-200.
@article{89f757a959bf4da7958ff419a4e7d5cc,
title = "Structure of the human CCG1 gene: relationship between the exons/introns and functional domain/modules of the protein",
abstract = "The human CCG1 gene, encoding CCGl/TAFII250/p250, was isolated by complementing tsBN462, a mutant BHK21 cell line that shows cell-cycle arrest at high temperature. Using the cDNA as a probe, the locations of exon-intron junctions were determined in the genomic DNA. Thirty-eight exons ranging from 68 to 219 bp in size were found. All the exonintron junctions followed the GT-AG rule. Using a newly developed method, we performed a module analysis of the CCG1 protein. The functional domain previously predicted in CCG1 was further confirmed to be encoded in a single predicted module that is the minimal functional unit in the protein. The boundaries of the predicted modules show a close correlation to the intron/exon junction of CCG1. The entire gene, at least 110kb long, has been recovered in a YAC, which provides a route to the further study of module function.",
keywords = "Cell cycle, TAF250, tsBN462 cells",
author = "Nakashima Torahiko and Sekiguchi Takeshi and Sunamoto Hidetoshi and Kei Yura and Tomoda Shirou and Go Mitiko and Kere Juha and Schlessinger David and Nishimoto Takeharu",
year = "1994",
month = "4",
day = "20",
doi = "10.1016/0378-1119(94)90570-3",
language = "English",
volume = "141",
pages = "193--200",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Structure of the human CCG1 gene

T2 - relationship between the exons/introns and functional domain/modules of the protein

AU - Torahiko, Nakashima

AU - Takeshi, Sekiguchi

AU - Hidetoshi, Sunamoto

AU - Yura, Kei

AU - Shirou, Tomoda

AU - Mitiko, Go

AU - Juha, Kere

AU - David, Schlessinger

AU - Takeharu, Nishimoto

PY - 1994/4/20

Y1 - 1994/4/20

N2 - The human CCG1 gene, encoding CCGl/TAFII250/p250, was isolated by complementing tsBN462, a mutant BHK21 cell line that shows cell-cycle arrest at high temperature. Using the cDNA as a probe, the locations of exon-intron junctions were determined in the genomic DNA. Thirty-eight exons ranging from 68 to 219 bp in size were found. All the exonintron junctions followed the GT-AG rule. Using a newly developed method, we performed a module analysis of the CCG1 protein. The functional domain previously predicted in CCG1 was further confirmed to be encoded in a single predicted module that is the minimal functional unit in the protein. The boundaries of the predicted modules show a close correlation to the intron/exon junction of CCG1. The entire gene, at least 110kb long, has been recovered in a YAC, which provides a route to the further study of module function.

AB - The human CCG1 gene, encoding CCGl/TAFII250/p250, was isolated by complementing tsBN462, a mutant BHK21 cell line that shows cell-cycle arrest at high temperature. Using the cDNA as a probe, the locations of exon-intron junctions were determined in the genomic DNA. Thirty-eight exons ranging from 68 to 219 bp in size were found. All the exonintron junctions followed the GT-AG rule. Using a newly developed method, we performed a module analysis of the CCG1 protein. The functional domain previously predicted in CCG1 was further confirmed to be encoded in a single predicted module that is the minimal functional unit in the protein. The boundaries of the predicted modules show a close correlation to the intron/exon junction of CCG1. The entire gene, at least 110kb long, has been recovered in a YAC, which provides a route to the further study of module function.

KW - Cell cycle

KW - TAF250

KW - tsBN462 cells

UR - http://www.scopus.com/inward/record.url?scp=0028221721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028221721&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(94)90570-3

DO - 10.1016/0378-1119(94)90570-3

M3 - Article

VL - 141

SP - 193

EP - 200

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -