Structure of the human CCG1 gene: relationship between the exons/introns and functional domain/modules of the protein

Nakashima Torahiko, Sekiguchi Takeshi, Sunamoto Hidetoshi, Yura Kei, Tomoda Shirou, Go Mitiko, Kere Juha, Schlessinger David, Nishimoto Takeharu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The human CCG1 gene, encoding CCGl/TAFII250/p250, was isolated by complementing tsBN462, a mutant BHK21 cell line that shows cell-cycle arrest at high temperature. Using the cDNA as a probe, the locations of exon-intron junctions were determined in the genomic DNA. Thirty-eight exons ranging from 68 to 219 bp in size were found. All the exonintron junctions followed the GT-AG rule. Using a newly developed method, we performed a module analysis of the CCG1 protein. The functional domain previously predicted in CCG1 was further confirmed to be encoded in a single predicted module that is the minimal functional unit in the protein. The boundaries of the predicted modules show a close correlation to the intron/exon junction of CCG1. The entire gene, at least 110kb long, has been recovered in a YAC, which provides a route to the further study of module function.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalGene
Volume141
Issue number2
DOIs
Publication statusPublished - 1994 Apr 20
Externally publishedYes

Keywords

  • Cell cycle
  • TAF250
  • tsBN462 cells

ASJC Scopus subject areas

  • Genetics

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    Torahiko, N., Takeshi, S., Hidetoshi, S., Kei, Y., Shirou, T., Mitiko, G., Juha, K., David, S., & Takeharu, N. (1994). Structure of the human CCG1 gene: relationship between the exons/introns and functional domain/modules of the protein. Gene, 141(2), 193-200. https://doi.org/10.1016/0378-1119(94)90570-3