Structure of the liposome composed of lipid-heme and phospholipids

Makoto Yuasa, Kazukiyo Aiba, Yoshitaka Ogata, Hiroyuki Nishide, Eishun Tsuchida

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11 Citations (Scopus)

Abstract

The amphiphilic heme derivative, 5,10,15,20-tetra(α,α,α,α-o-(2′,2′-dimethyl-20′-(2′-trimethylammonioethyl) phosphonatoxyicosanamido)pheny)phorphinatoiron(II) (lipid-heme), formed a stable liposome (Φ ≈ 400 Å) with phospholipids. Differential scanning calorimetry showed that incorporation of the lipid-heme in the liposome bilayer (lipid/lipid-heme > 25) causes no disordering of the bilayer structure. Ligation of a bulky ligand to the lipid-heme liposome indicated that the lipid-heme situates facing predominantly outwards in the liposome. The closed vesicle structure and the stability of the lipid-heme liposome were also confirmed by the encapsulating capability of the fluorescence compound.

Original languageEnglish
Pages (from-to)558-565
Number of pages8
JournalBBA - Biomembranes
Volume860
Issue number3
DOIs
Publication statusPublished - 1986 Sep 11

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Keywords

  • Heme
  • Hemoglobin model
  • Lipid-heme
  • Liposome
  • Oxygen carrier

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Yuasa, M., Aiba, K., Ogata, Y., Nishide, H., & Tsuchida, E. (1986). Structure of the liposome composed of lipid-heme and phospholipids. BBA - Biomembranes, 860(3), 558-565. https://doi.org/10.1016/0005-2736(86)90553-5