Structure of the Microtubule-Binding Domain of Flagellar Dynein

Yusuke S. Kato, Toshiki Yagi, Sarah A. Harris, Shin Ya Ohki, Kei Yura, Youské Shimizu, Shinya Honda, Ritsu Kamiya, Stan A. Burgess, Masaru Tanokura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Flagellar dyneins are essential microtubule motors in eukaryotes, as they drive the beating motions of cilia and flagella. Unlike myosin and kinesin motors, the track binding mechanism of dyneins and the regulation between the strong and weak binding states remain obscure. Here we report the solution structure of the microtubule-binding domain of flagellar dynein-c/DHC9 (dynein-c MTBD). The structure reveals a similar overall helix-rich fold to that of the MTBD of cytoplasmic dynein (cytoplasmic MTBD), but dynein-c MTBD has an additional flap, consisting of an antiparallel β sheet. The flap is positively charged and highly flexible. Despite the structural similarity to cytoplasmic MTBD, dynein-c MTBD shows only a small change in the microtubule-binding affinity depending on the registry change of coiled coil-sliding, whereby lacks the apparent strong binding state. The surface charge distribution of dynein-c MTBD also differs from that of cytoplasmic MTBD, which suggests a difference in the microtubule-binding mechanism.

Original languageEnglish
Pages (from-to)1628-1638
Number of pages11
JournalStructure
Volume22
Issue number11
DOIs
Publication statusPublished - 2014 Nov 4
Externally publishedYes

Fingerprint

Dyneins
Microtubules
Cytoplasmic Dyneins
Kinesin
Flagella
Cilia
Myosins
Eukaryota
Registries

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Kato, Y. S., Yagi, T., Harris, S. A., Ohki, S. Y., Yura, K., Shimizu, Y., ... Tanokura, M. (2014). Structure of the Microtubule-Binding Domain of Flagellar Dynein. Structure, 22(11), 1628-1638. https://doi.org/10.1016/j.str.2014.08.021

Structure of the Microtubule-Binding Domain of Flagellar Dynein. / Kato, Yusuke S.; Yagi, Toshiki; Harris, Sarah A.; Ohki, Shin Ya; Yura, Kei; Shimizu, Youské; Honda, Shinya; Kamiya, Ritsu; Burgess, Stan A.; Tanokura, Masaru.

In: Structure, Vol. 22, No. 11, 04.11.2014, p. 1628-1638.

Research output: Contribution to journalArticle

Kato, YS, Yagi, T, Harris, SA, Ohki, SY, Yura, K, Shimizu, Y, Honda, S, Kamiya, R, Burgess, SA & Tanokura, M 2014, 'Structure of the Microtubule-Binding Domain of Flagellar Dynein', Structure, vol. 22, no. 11, pp. 1628-1638. https://doi.org/10.1016/j.str.2014.08.021
Kato YS, Yagi T, Harris SA, Ohki SY, Yura K, Shimizu Y et al. Structure of the Microtubule-Binding Domain of Flagellar Dynein. Structure. 2014 Nov 4;22(11):1628-1638. https://doi.org/10.1016/j.str.2014.08.021
Kato, Yusuke S. ; Yagi, Toshiki ; Harris, Sarah A. ; Ohki, Shin Ya ; Yura, Kei ; Shimizu, Youské ; Honda, Shinya ; Kamiya, Ritsu ; Burgess, Stan A. ; Tanokura, Masaru. / Structure of the Microtubule-Binding Domain of Flagellar Dynein. In: Structure. 2014 ; Vol. 22, No. 11. pp. 1628-1638.
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