Structure, orientation and stability of lysozyme confined in layered materials

Sébastien Balme, Regis Guegan, Jean Marc Janot, Maguy Jaber, Mathilde Lepoitevin, Philippe Dejardin, Xavier Bourrat, Mikael Motelica-Heino

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The confinement of lysozyme in 3 layered materials based on montmorillonite and lamellar double hydroxides exhibiting different surface charges was studied. The protein structure and orientation in these materials were determined by X-ray diffraction, time resolved fluorescence and fluorescence anisotropy. For montmorillonite exchanged with sodium and modified with a non-ionic surfactant (tri-ethylene glycol mono n-decyl ether), the lysozyme was found to be located in the interlayer space with the "end-on" and "side-on" orientations, respectively. Conversely, no lysozyme intercalation was observed with a lamellar double hydroxide modified with an anionic surfactant (sodium octylsulfate), since the protein was adsorbed on the surface of the particles. Fourier transformed infrared spectroscopy analysis shows that lysozyme confinement in the interlayer space preserves its structure after dehydration, whereas some structural changes were observed for lysozyme adsorbed on the particle surface.

Original languageEnglish
Pages (from-to)3188-3196
Number of pages9
JournalSoft Matter
Volume9
Issue number11
DOIs
Publication statusPublished - 2013 Mar 21
Externally publishedYes

Fingerprint

lysozyme
Muramidase
Bentonite
montmorillonite
hydroxides
interlayers
Hydroxides
Sodium
Fluorescence
surfactants
sodium
proteins
fluorescence
Ethylene Glycol
Anionic surfactants
Nonionic surfactants
Surface charge
Intercalation
Dehydration
intercalation

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Balme, S., Guegan, R., Janot, J. M., Jaber, M., Lepoitevin, M., Dejardin, P., ... Motelica-Heino, M. (2013). Structure, orientation and stability of lysozyme confined in layered materials. Soft Matter, 9(11), 3188-3196. https://doi.org/10.1039/c3sm27880h

Structure, orientation and stability of lysozyme confined in layered materials. / Balme, Sébastien; Guegan, Regis; Janot, Jean Marc; Jaber, Maguy; Lepoitevin, Mathilde; Dejardin, Philippe; Bourrat, Xavier; Motelica-Heino, Mikael.

In: Soft Matter, Vol. 9, No. 11, 21.03.2013, p. 3188-3196.

Research output: Contribution to journalArticle

Balme, S, Guegan, R, Janot, JM, Jaber, M, Lepoitevin, M, Dejardin, P, Bourrat, X & Motelica-Heino, M 2013, 'Structure, orientation and stability of lysozyme confined in layered materials', Soft Matter, vol. 9, no. 11, pp. 3188-3196. https://doi.org/10.1039/c3sm27880h
Balme S, Guegan R, Janot JM, Jaber M, Lepoitevin M, Dejardin P et al. Structure, orientation and stability of lysozyme confined in layered materials. Soft Matter. 2013 Mar 21;9(11):3188-3196. https://doi.org/10.1039/c3sm27880h
Balme, Sébastien ; Guegan, Regis ; Janot, Jean Marc ; Jaber, Maguy ; Lepoitevin, Mathilde ; Dejardin, Philippe ; Bourrat, Xavier ; Motelica-Heino, Mikael. / Structure, orientation and stability of lysozyme confined in layered materials. In: Soft Matter. 2013 ; Vol. 9, No. 11. pp. 3188-3196.
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