Structure–activity relationships and action mechanisms of collagen-like antimicrobial peptides

Ryo Masuda, Yui Dazai, Takehiko Mima, Takaki Koide

    Research output: Contribution to journalArticle

    3 Citations (Scopus)


    An antimicrobial triple-helical peptide, R3, was previously obtained from a collagen-like combinatorial peptide library. In this research, based on structure–activity relationship studies of R3, a more potent peptide, RR4, with increased positive net charge and charge density relative to R3, was developed. RR4 exhibited antimicrobial activity against both Gram-negative and Gram-positive bacterial strains, including multidrug-resistant strains. Its action could be attributed to entry into cells and interactions with intercellular molecules such as DNA/RNA that inhibited cell division rather than increasing bacterial membrane permeability. Furthermore, RR4 exhibited remarkable stability in serum and low cytotoxicity.

    Original languageEnglish
    Article numbere22931
    Issue number1
    Publication statusPublished - 2017 Jan 1



    • antimicrobial peptide
    • collagen
    • structure–activity relationship
    • triple helix

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Biomaterials
    • Organic Chemistry

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