Structures of human nucleosomes containing major histone H3 variants

Hiroaki Tachiwana, Akihisa Osakabe, Tatsuya Shiga, Yuta Miya, Hiroshi Kimura, Wataru Kagawa, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    58 Citations (Scopus)

    Abstract

    The nucleosome is the fundamental repeating unit of chromatin, via which genomic DNA is packaged into the nucleus in eukaryotes. In the nucleosome, two copies of each core histone, H2A, H2B, H3 and H4, form a histone octamer which wraps 146 base pairs of DNA around itself. All of the core histones except for histone H4 have nonallelic isoforms called histone variants. In humans, eight histone H3 variants, H3.1, H3.2, H3.3, H3T, H3.5, H3.X, H3.Y and CENP-A, have been reported to date. Previous studies have suggested that histone H3 variants possess distinct functions in the formation of specific chromosome regions and/or in the regulation of transcription and replication. H3.1, H3.2 and H3.3 are the most abundant H3 variants. Here, crystal structures of human nucleosomes containing either H3.2 or H3.3 have been solved. The structures were essentially the same as that of the H3.1 nucleosome. Since the amino-acid residues specific for H3.2 and H3.3 are located on the accessible surface of the H3/H4 tetramer, they may be potential interaction sites for H3.2- and H3.3-specific chaperones.

    Original languageEnglish
    Pages (from-to)578-583
    Number of pages6
    JournalActa Crystallographica Section D: Biological Crystallography
    Volume67
    Issue number6
    DOIs
    Publication statusPublished - 2011 Jun

    Fingerprint

    Nucleosomes
    Histones
    DNA
    Eukaryota
    Base Pairing
    Chromatin
    Protein Isoforms
    Chromosomes
    Amino Acids

    Keywords

    • chromatin
    • H3
    • histone variants
    • nucleosomes

    ASJC Scopus subject areas

    • Structural Biology

    Cite this

    Tachiwana, H., Osakabe, A., Shiga, T., Miya, Y., Kimura, H., Kagawa, W., & Kurumizaka, H. (2011). Structures of human nucleosomes containing major histone H3 variants. Acta Crystallographica Section D: Biological Crystallography, 67(6), 578-583. https://doi.org/10.1107/S0907444911014818

    Structures of human nucleosomes containing major histone H3 variants. / Tachiwana, Hiroaki; Osakabe, Akihisa; Shiga, Tatsuya; Miya, Yuta; Kimura, Hiroshi; Kagawa, Wataru; Kurumizaka, Hitoshi.

    In: Acta Crystallographica Section D: Biological Crystallography, Vol. 67, No. 6, 06.2011, p. 578-583.

    Research output: Contribution to journalArticle

    Tachiwana, H, Osakabe, A, Shiga, T, Miya, Y, Kimura, H, Kagawa, W & Kurumizaka, H 2011, 'Structures of human nucleosomes containing major histone H3 variants', Acta Crystallographica Section D: Biological Crystallography, vol. 67, no. 6, pp. 578-583. https://doi.org/10.1107/S0907444911014818
    Tachiwana, Hiroaki ; Osakabe, Akihisa ; Shiga, Tatsuya ; Miya, Yuta ; Kimura, Hiroshi ; Kagawa, Wataru ; Kurumizaka, Hitoshi. / Structures of human nucleosomes containing major histone H3 variants. In: Acta Crystallographica Section D: Biological Crystallography. 2011 ; Vol. 67, No. 6. pp. 578-583.
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