STT3, a novel essential gene related to the PKC1/STT1 protein kinase pathway, is involved in protein glycosylation in yeast

Satoshi Yoshida, Yoshikazu Ohya, Akihiko Nakano, Yasuhiro Anraku

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34 Citations (Scopus)

Abstract

Mutations of genes involved in the STT1/PKC1 pathway in yeast show staurosporine and temperature sensitivities (stt) which are suppressed by the addition of 1 M sorbitol [Yoshida et al., Mol. Gen. Genet. 242 (1994) 631-640]. Among the stt mutants, stt3-2 shares this phenotype. The STT3 gene encodes a novel 718-amino-acid protein with significant homology to potential transmembrane proteins of Caenorhabditis elegans and mouse mandibular condyle (about 80% homologous and 60% identical). Unlike the STT1/PKC1 gene, STT3 is essential for cell growth irrespective of osmotic support. Pulse-chase experiments show that the stt3 mutants are defective in protein glycosylation. The stt3 mutants are sensitive to hygromycin B and resistant to sodium orthovanadate, whose phenotypes are common to those defective in protein glycosylation.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalGene
Volume164
Issue number1
DOIs
Publication statusPublished - 1995 Oct 16

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Keywords

  • Saccharomyces cerevisiae
  • protein kinase C
  • sorbitol
  • staurosporine sensitivity
  • transmembrane protein
  • vanadate resistance

ASJC Scopus subject areas

  • Genetics

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