TY - JOUR
T1 - Studies on the F-actin · tropomyosin · troponin complex III. Effects of troponin components and calcium ion on the binding affinity between tropomyosin and F-actin
AU - Ishiwata, Shin'ichi
PY - 1978/6/21
Y1 - 1978/6/21
N2 - We studied the effects of the troponin components TN-T, TN-I and TN-C, and of Ca2+ concentration on the dissociation temperature of the F-actin · tropomyosin complex. Troponin components and their complexes increased the dissociation temperature in the following sequence of effectiveness: TN-C(±Ca2+) < TN-I < TN-I · TN-C(±Ca2+) ≪ TN-T · TN-C(+Ca2+) ≪ TN-T · TN-C(-Ca2+) ≈ TN-T < TN-T · TN-I · TN-C(+Ca2+) < TN-T · TN-I · TN-C-(-Ca2+) ≈ TN-T · TN-I; TN-C has no effect. The effect of TN-I is small. TN-T is the most important component for increasing the dissociation temperature. The shifting by Ca2+ of the dissociation temperature to lower values is only seen when both TN-T and TN-C are present.
AB - We studied the effects of the troponin components TN-T, TN-I and TN-C, and of Ca2+ concentration on the dissociation temperature of the F-actin · tropomyosin complex. Troponin components and their complexes increased the dissociation temperature in the following sequence of effectiveness: TN-C(±Ca2+) < TN-I < TN-I · TN-C(±Ca2+) ≪ TN-T · TN-C(+Ca2+) ≪ TN-T · TN-C(-Ca2+) ≈ TN-T < TN-T · TN-I · TN-C(+Ca2+) < TN-T · TN-I · TN-C-(-Ca2+) ≈ TN-T · TN-I; TN-C has no effect. The effect of TN-I is small. TN-T is the most important component for increasing the dissociation temperature. The shifting by Ca2+ of the dissociation temperature to lower values is only seen when both TN-T and TN-C are present.
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U2 - 10.1016/0005-2795(78)90018-1
DO - 10.1016/0005-2795(78)90018-1
M3 - Article
C2 - 667109
AN - SCOPUS:0017849685
SN - 1570-9639
VL - 534
SP - 350
EP - 357
JO - BBA - Protein Structure
JF - BBA - Protein Structure
IS - 2
ER -