Study of folding/unfolding kinetics of lattice proteins by applying a simple statistical mechanical model for protein folding

Hiroshi Wako, Haruo Abe

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The folding/unfolding kinetics of a three-dimensional lattice protein was studied using a simple statistical mechanical model for protein folding that we had developed earlier. The model considers the specificity of an amino acid sequence and the native structure of a given protein. We calculated the characteristic relaxation rate on the free energy surface starting from a completely unfolded structure (or native structure) that is assumed to associate with a folding rate (or an unfolding rate). The chevron plot of these rates as a function of the inverse temperature was obtained for four lattice proteins, a1, a2, b1, and b2, in order to investigate the dependency of the folding and unfolding rates on their native structures and amino acid sequences. Proteins a1 and a2 fold to the same native structure, but their amino acid sequences differ. The same is true for proteins b1 and b2, but their native structure is different from that of a1 and a2. To elucidate the roles of individual amino acid residues in protein folding/unfolding kinetics, we calculated the kinetic properties for all possible single amino acid substitutions of these proteins and examined their responses. The results are discussed with respect to the roles of short- and long-range interactions and formation of a folding nucleus in the kinetics of protein folding/unfolding.

Original languageEnglish
Title of host publicationNew Developments in Proteomics Research
PublisherNova Science Publishers, Inc.
Pages55-82
Number of pages28
ISBN (Print)9781619422292
Publication statusPublished - 2012 Jan

Fingerprint

Protein folding
Protein Folding
Statistical Models
Kinetics
Amino Acids
Amino Acid Sequence
Protein Unfolding
Proteins
Amino Acid Substitution
Free energy
Substitution reactions
Temperature

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wako, H., & Abe, H. (2012). Study of folding/unfolding kinetics of lattice proteins by applying a simple statistical mechanical model for protein folding. In New Developments in Proteomics Research (pp. 55-82). Nova Science Publishers, Inc..

Study of folding/unfolding kinetics of lattice proteins by applying a simple statistical mechanical model for protein folding. / Wako, Hiroshi; Abe, Haruo.

New Developments in Proteomics Research. Nova Science Publishers, Inc., 2012. p. 55-82.

Research output: Chapter in Book/Report/Conference proceedingChapter

Wako, H & Abe, H 2012, Study of folding/unfolding kinetics of lattice proteins by applying a simple statistical mechanical model for protein folding. in New Developments in Proteomics Research. Nova Science Publishers, Inc., pp. 55-82.
Wako H, Abe H. Study of folding/unfolding kinetics of lattice proteins by applying a simple statistical mechanical model for protein folding. In New Developments in Proteomics Research. Nova Science Publishers, Inc. 2012. p. 55-82
Wako, Hiroshi ; Abe, Haruo. / Study of folding/unfolding kinetics of lattice proteins by applying a simple statistical mechanical model for protein folding. New Developments in Proteomics Research. Nova Science Publishers, Inc., 2012. pp. 55-82
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