Study on Some Factors Affecting the Precision of Luminescence Analyses

Akiyoshi Taniguchi, Hidetaka Yuki, Yuzuru Hayashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effects of the shape of the reaction vessel, the sample volume and the volume and speed of the reagent injection on the reproducibility of the chemiluminescence (CL) analyses were investigated with a laboratory-built automated injection device and a computer system. The suitable selections of the vessel diameter as well as the injection volume are shown to be necessary for the precise measurements of samples of given volume. Under the optimal mixing conditions, the choice of signal processings such as peak height measurements or fixed-time integration did not affect the reproducibility and any of the methods used could give accurate CL values. These principles for the precise measurement represent useful guides for luminometer designing and experiments for rapid, precise analyses.

Original languageEnglish
Pages (from-to)3475-3480
Number of pages6
JournalChemical and Pharmaceutical Bulletin
Volume34
Issue number8
DOIs
Publication statusPublished - 1986
Externally publishedYes

Fingerprint

Luminescence
Chemiluminescence
Injections
Computer Systems
Signal processing
Computer systems
Equipment and Supplies
Experiments

Keywords

  • automated injection
  • chemiluminescence
  • computer analysis
  • luminescence
  • mixing mode

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

Cite this

Study on Some Factors Affecting the Precision of Luminescence Analyses. / Taniguchi, Akiyoshi; Yuki, Hidetaka; Hayashi, Yuzuru.

In: Chemical and Pharmaceutical Bulletin, Vol. 34, No. 8, 1986, p. 3475-3480.

Research output: Contribution to journalArticle

Taniguchi, Akiyoshi ; Yuki, Hidetaka ; Hayashi, Yuzuru. / Study on Some Factors Affecting the Precision of Luminescence Analyses. In: Chemical and Pharmaceutical Bulletin. 1986 ; Vol. 34, No. 8. pp. 3475-3480.
@article{ac9ea573ed8b412aaf07c4ad99bbd1fd,
title = "Study on Some Factors Affecting the Precision of Luminescence Analyses",
abstract = "The effects of the shape of the reaction vessel, the sample volume and the volume and speed of the reagent injection on the reproducibility of the chemiluminescence (CL) analyses were investigated with a laboratory-built automated injection device and a computer system. The suitable selections of the vessel diameter as well as the injection volume are shown to be necessary for the precise measurements of samples of given volume. Under the optimal mixing conditions, the choice of signal processings such as peak height measurements or fixed-time integration did not affect the reproducibility and any of the methods used could give accurate CL values. These principles for the precise measurement represent useful guides for luminometer designing and experiments for rapid, precise analyses.",
keywords = "automated injection, chemiluminescence, computer analysis, luminescence, mixing mode",
author = "Akiyoshi Taniguchi and Hidetaka Yuki and Yuzuru Hayashi",
year = "1986",
doi = "10.1248/cpb.34.3475",
language = "English",
volume = "34",
pages = "3475--3480",
journal = "Chemical and Pharmaceutical Bulletin",
issn = "0009-2363",
publisher = "Pharmaceutical Society of Japan",
number = "8",

}

TY - JOUR

T1 - Study on Some Factors Affecting the Precision of Luminescence Analyses

AU - Taniguchi, Akiyoshi

AU - Yuki, Hidetaka

AU - Hayashi, Yuzuru

PY - 1986

Y1 - 1986

N2 - The effects of the shape of the reaction vessel, the sample volume and the volume and speed of the reagent injection on the reproducibility of the chemiluminescence (CL) analyses were investigated with a laboratory-built automated injection device and a computer system. The suitable selections of the vessel diameter as well as the injection volume are shown to be necessary for the precise measurements of samples of given volume. Under the optimal mixing conditions, the choice of signal processings such as peak height measurements or fixed-time integration did not affect the reproducibility and any of the methods used could give accurate CL values. These principles for the precise measurement represent useful guides for luminometer designing and experiments for rapid, precise analyses.

AB - The effects of the shape of the reaction vessel, the sample volume and the volume and speed of the reagent injection on the reproducibility of the chemiluminescence (CL) analyses were investigated with a laboratory-built automated injection device and a computer system. The suitable selections of the vessel diameter as well as the injection volume are shown to be necessary for the precise measurements of samples of given volume. Under the optimal mixing conditions, the choice of signal processings such as peak height measurements or fixed-time integration did not affect the reproducibility and any of the methods used could give accurate CL values. These principles for the precise measurement represent useful guides for luminometer designing and experiments for rapid, precise analyses.

KW - automated injection

KW - chemiluminescence

KW - computer analysis

KW - luminescence

KW - mixing mode

UR - http://www.scopus.com/inward/record.url?scp=85004235248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85004235248&partnerID=8YFLogxK

U2 - 10.1248/cpb.34.3475

DO - 10.1248/cpb.34.3475

M3 - Article

VL - 34

SP - 3475

EP - 3480

JO - Chemical and Pharmaceutical Bulletin

JF - Chemical and Pharmaceutical Bulletin

SN - 0009-2363

IS - 8

ER -