Subcutaneous transplantation of autologous oral mucosal epithelial cell sheets fabricated on temperature-responsive culture dishes

Haruko Obokata, Masayuki Yamato, Joseph Yang, Kohji Nishida, Satoshi Tsuneda, Teruo Okano

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The oral mucosa is an attractive cell source for autologous transplantation in human patients who require regenerative therapies of various epithelia. However, the time-course of cellular changes in transplanted oral mucosal epithelia at ectopic sites remains poorly understood. By applying a rat model, we analyzed phenotypic changes in oral mucosal epithelial cell sheets after harvest from temperature-responsive culture dishes and subsequent autologous subcutaneous transplantation. We used monoclonal antibodies to identify epithelial-specific cytokeratins 4, 10, 13, and 14, the stem/progenitor cell marker p63, and proliferating cell nuclear antigen, within the regenerated tissues. Transplanted oral mucosal epithelial cell sheets proliferated during the first week after grafting in conjunction with host inflammation, but then began to degenerate afterward with complete disappearance after 3 weeks. Our findings suggest that host subcutaneous tissues support proliferation and differentiation of the oral mucosal epithelial cell sheets, but are unable to promote maintenance of stem and progenitor cells and therefore cannot produce long-term survivability.

    Original languageEnglish
    Pages (from-to)1088-1096
    Number of pages9
    JournalJournal of Biomedical Materials Research - Part A
    Volume86
    Issue number4
    DOIs
    Publication statusPublished - 2008 Sep 15

    Fingerprint

    Keratin-4
    Tissue
    Monoclonal antibodies
    Proliferating Cell Nuclear Antigen
    Antigens
    Stem cells
    Temperature
    Rats
    Monoclonal Antibodies
    Epithelial Cells
    Mucous Membrane

    Keywords

    • Cell sheet
    • Phenotypic modulation
    • Progenitor cell
    • Tissue engineering autologous cell

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Biomaterials

    Cite this

    Subcutaneous transplantation of autologous oral mucosal epithelial cell sheets fabricated on temperature-responsive culture dishes. / Obokata, Haruko; Yamato, Masayuki; Yang, Joseph; Nishida, Kohji; Tsuneda, Satoshi; Okano, Teruo.

    In: Journal of Biomedical Materials Research - Part A, Vol. 86, No. 4, 15.09.2008, p. 1088-1096.

    Research output: Contribution to journalArticle

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    AU - Okano, Teruo

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