Submicrosecond Imaging Under a Pulsed-Laser Fluorescence Microscope

Kazuhiko Kinosita, Ikuo Ashikawa, Masahiro Hibino, Akira Lkegami

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A microscope system has been constructed that enables digital imaging of a fluorescent cell under pulsed illumination. Each image is produced by a single laser pulse of duration less than 0.3 µ s. With this system, microsecond responses of a single cell to an externally applied electric field have been resolved temporally and spatially. The cell membrane was stained with a voltage-sensitive fluorescent dye. The induction of transsmembrane potential by the applied field, and the perforation (electroporation) of the cell membrane under an intense field, were seen as successive images. The major finding was a transient increase, at the moment of perforation, in the membrane permeability to an enormous level in localized regions of the cell membrane. Possible roles in cell technology, as well as other applications of the microscope system, are discussed.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume909
DOIs
Publication statusPublished - 1988 Jun 24
Externally publishedYes

Fingerprint

Pulsed Laser
Cell membranes
Pulsed lasers
Microscope
Fluorescence
pulsed lasers
perforation
Microscopes
microscopes
Imaging
Imaging techniques
fluorescence
Cell
Membrane
cells
Fluorescent Dyes
Laser pulses
induction
permeability
Dyes

ASJC Scopus subject areas

  • Applied Mathematics
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering
  • Computer Science Applications

Cite this

Submicrosecond Imaging Under a Pulsed-Laser Fluorescence Microscope. / Kinosita, Kazuhiko; Ashikawa, Ikuo; Hibino, Masahiro; Lkegami, Akira.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 909, 24.06.1988, p. 271-277.

Research output: Contribution to journalArticle

Kinosita, Kazuhiko ; Ashikawa, Ikuo ; Hibino, Masahiro ; Lkegami, Akira. / Submicrosecond Imaging Under a Pulsed-Laser Fluorescence Microscope. In: Proceedings of SPIE - The International Society for Optical Engineering. 1988 ; Vol. 909. pp. 271-277.
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