Subzero temperature operating biosensor utilizing an organic solvent and quinoprotein glucose dehydrogenase

Koji Sode, Satoshi Nakasono, Mitsuharu Tanaka, Tadashi Matsunaga

Research output: Contribution to journalLetterpeer-review

27 Citations (Scopus)

Abstract

A subzero temperature operating biosensor was constructed using immobilized quinoprotein glucose dehydrogenase (PQQGDH), glassy carbon electrode, soluble electron mediator (ferrocene monocarboxylic acid), and an organic solvent, ethylene glycol, as an antifreezing reagent. Using this biosensor, glucose concentration can be determined even at −7°C. At this temperature, the response was 20% of that obtained at 20°C. This is the first study describing a subzero temperature operating biosensor. © 1993 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)251-254
Number of pages4
JournalBiotechnology and Bioengineering
Volume42
Issue number2
DOIs
Publication statusPublished - 1993
Externally publishedYes

Keywords

  • biosensor
  • PQQGDH
  • subzero temperature

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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