Suppression effect of methemoglobin formation in hemoglobin vesicles by coencapsulating methemoglobin and L-tyrosine

Tomoyasu Atoji; Motonari Aihara; Shinji Takeoka; Eishun Tsuchida

Suppression effect of methemoglobin formation in hemoglobin vesicles by coencapsulating methemoglobin and L-tyrosine

Tomoyasu Atoji, Motonari Aihara, Shinji Takeoka, Eishun Tsuchida

School of Advanced Science and Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

Oxyhemoglobin(oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide(H ₂O ₂) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. In this study, we constructed a suppression system of metHb formation within Hb-vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb solution by adding metHb/L-Tyr in the experiment of stepwise addition of H ₂O ₂. We also confirmed similar effect in Hb vesicles coencapsulating metHb/L-Tyr. Furthermore, it was also effective in vivo. We concluded that H ₂O ₂ was stably eliminated by the metHb/L-Tyr system in Hb-vesicles, and oxyHb was protected from H ₂O ₂ reaction.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Conference

Conference	54th SPSJ Annual Meeting 2005
Country	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

Keywords

Hb-vesicles
Hydrogen peroxide
L-tyrosine
MetHb
Peroxidase activity

ASJC Scopus subject areas

Engineering(all)

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title = "Suppression effect of methemoglobin formation in hemoglobin vesicles by coencapsulating methemoglobin and L-tyrosine",

abstract = "Oxyhemoglobin(oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide(H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. In this study, we constructed a suppression system of metHb formation within Hb-vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb solution by adding metHb/L-Tyr in the experiment of stepwise addition of H 2O 2. We also confirmed similar effect in Hb vesicles coencapsulating metHb/L-Tyr. Furthermore, it was also effective in vivo. We concluded that H 2O 2 was stably eliminated by the metHb/L-Tyr system in Hb-vesicles, and oxyHb was protected from H 2O 2 reaction.",

keywords = "Hb-vesicles, Hydrogen peroxide, L-tyrosine, MetHb, Peroxidase activity",

author = "Tomoyasu Atoji and Motonari Aihara and Shinji Takeoka and Eishun Tsuchida",

year = "2005",

month = dec,

day = "1",

language = "English",

note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

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TY - CONF

T1 - Suppression effect of methemoglobin formation in hemoglobin vesicles by coencapsulating methemoglobin and L-tyrosine

AU - Atoji, Tomoyasu

AU - Aihara, Motonari

AU - Takeoka, Shinji

AU - Tsuchida, Eishun

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Oxyhemoglobin(oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide(H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. In this study, we constructed a suppression system of metHb formation within Hb-vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb solution by adding metHb/L-Tyr in the experiment of stepwise addition of H 2O 2. We also confirmed similar effect in Hb vesicles coencapsulating metHb/L-Tyr. Furthermore, it was also effective in vivo. We concluded that H 2O 2 was stably eliminated by the metHb/L-Tyr system in Hb-vesicles, and oxyHb was protected from H 2O 2 reaction.

AB - Oxyhemoglobin(oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide(H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. In this study, we constructed a suppression system of metHb formation within Hb-vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb solution by adding metHb/L-Tyr in the experiment of stepwise addition of H 2O 2. We also confirmed similar effect in Hb vesicles coencapsulating metHb/L-Tyr. Furthermore, it was also effective in vivo. We concluded that H 2O 2 was stably eliminated by the metHb/L-Tyr system in Hb-vesicles, and oxyHb was protected from H 2O 2 reaction.

KW - Hb-vesicles

KW - Hydrogen peroxide

KW - L-tyrosine

KW - MetHb

KW - Peroxidase activity

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T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

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