Suppression of metHb formation in Hb-vesicles by glutathione

H. Yokohama, Y. Seino, J. E. Chung, H. Sakai, S. Takeoka, H. Nishide, E. Tsuchida

Research output: Contribution to journalArticle

Abstract

To increase the amount of oxygen transported by Hb-vesicles, it is important to suppress metHb formation. α-Tocopherol decreased lipid peroxidation in Hb-vesicles composed of EYL, whereas the Hb-vesicles composed of saturated phospholipids, such as DPPC, showed less metHb formation. To suppress metHb formation in a highly purified Hb solution, glutathione (GSH) was studied as a reductant. GSH is oxidized not only by metHb reduction but also by O2 to generate active oxygen species, which react with Hb to form metHb. Effective nonenzymatic metHb reduction was observed at a high [Hb] because of the acceleration of metHb reduction, and at lower partial pressure of O2 (pO2) because of suppression of GSH oxidation. Hb-vesicles encapsulating 40 g/dl Hb with pyridoxal 5'-phosphate showed high oxygen transporting efficiency. The rate of metHb formation at a low pO2 in the blood stream was decreased by GSH.

Original languageEnglish
Pages (from-to)868-871
Number of pages4
JournalJapanese Journal of Artificial Organs
Volume23
Issue number3
Publication statusPublished - 1994 Jan 1

Keywords

  • Hb-vesicles
  • glutathione
  • lipid peroxidation
  • metHb reduction
  • α-tocopherol

ASJC Scopus subject areas

  • Biophysics

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    Yokohama, H., Seino, Y., Chung, J. E., Sakai, H., Takeoka, S., Nishide, H., & Tsuchida, E. (1994). Suppression of metHb formation in Hb-vesicles by glutathione. Japanese Journal of Artificial Organs, 23(3), 868-871.