Suppression of neuroinflammation in forebrain-specific Cdk5 conditional knockout mice by PPARγ agonist improves neuronal loss and early lethality

Elias Utreras, Ryusuke Hamada, Michaela Prochazkova, Anita Terse, Satoru Takahashi, Toshio Ohshima, Ashok B. Kulkarni

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Background: Cyclin-dependent kinase 5 (Cdk5) is essential for brain development and function, and its deregulated expression is implicated in some of neurodegenerative diseases. We reported earlier that the forebrain-specific Cdk5 conditional knockout (cKO) mice displayed an early lethality associated with neuroinflammation, increased expression of the neuronal tissue-type plasminogen activator (tPA), and neuronal migration defects.Methods: In order to suppress neuroinflammation in the cKO mice, we first treated these mice with pioglitazone, a PPARγ agonist, and analyzed its effects on neuronal loss and longevity. In a second approach, to delineate the precise role of tPA in neuroinflammation in these mice, we generated Cdk5 cKO; tPA double knockout (dKO) mice.Results: We found that pioglitazone treatment significantly reduced astrogliosis, microgliosis, neuronal loss and behavioral deficit in Cdk5 cKO mice. Interestingly, the dKO mice displayed a partial reversal in astrogliosis, but they still died at early age, suggesting that the increased expression of tPA in the cKO mice does not contribute significantly to the pathological process leading to neuroinflammation, neuronal loss and early lethality.Conclusion: The suppression of neuroinflammation in Cdk5 cKO mice ameliorates gliosis and neuronal loss, thus suggesting the potential beneficial effects of the PPARγ agonist pioglitazone for the treatment for neurodegenerative diseases.

    Original languageEnglish
    Article number28
    JournalJournal of Neuroinflammation
    Volume11
    DOIs
    Publication statusPublished - 2014 Feb 5

    Fingerprint

    Cyclin-Dependent Kinase 5
    Peroxisome Proliferator-Activated Receptors
    Prosencephalon
    pioglitazone
    Knockout Mice
    Plasminogen Activators
    Neurodegenerative Diseases
    Gliosis
    Tissue Plasminogen Activator
    Pathologic Processes
    Brain

    Keywords

    • Cdk5
    • Cdk5 conditional knockout mice
    • Neuroinflammation
    • Pioglitazone
    • tPA

    ASJC Scopus subject areas

    • Cellular and Molecular Neuroscience
    • Neurology
    • Immunology
    • Neuroscience(all)

    Cite this

    Suppression of neuroinflammation in forebrain-specific Cdk5 conditional knockout mice by PPARγ agonist improves neuronal loss and early lethality. / Utreras, Elias; Hamada, Ryusuke; Prochazkova, Michaela; Terse, Anita; Takahashi, Satoru; Ohshima, Toshio; Kulkarni, Ashok B.

    In: Journal of Neuroinflammation, Vol. 11, 28, 05.02.2014.

    Research output: Contribution to journalArticle

    Utreras, Elias ; Hamada, Ryusuke ; Prochazkova, Michaela ; Terse, Anita ; Takahashi, Satoru ; Ohshima, Toshio ; Kulkarni, Ashok B. / Suppression of neuroinflammation in forebrain-specific Cdk5 conditional knockout mice by PPARγ agonist improves neuronal loss and early lethality. In: Journal of Neuroinflammation. 2014 ; Vol. 11.
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    AU - Terse, Anita

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    AU - Kulkarni, Ashok B.

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    AB - Background: Cyclin-dependent kinase 5 (Cdk5) is essential for brain development and function, and its deregulated expression is implicated in some of neurodegenerative diseases. We reported earlier that the forebrain-specific Cdk5 conditional knockout (cKO) mice displayed an early lethality associated with neuroinflammation, increased expression of the neuronal tissue-type plasminogen activator (tPA), and neuronal migration defects.Methods: In order to suppress neuroinflammation in the cKO mice, we first treated these mice with pioglitazone, a PPARγ agonist, and analyzed its effects on neuronal loss and longevity. In a second approach, to delineate the precise role of tPA in neuroinflammation in these mice, we generated Cdk5 cKO; tPA double knockout (dKO) mice.Results: We found that pioglitazone treatment significantly reduced astrogliosis, microgliosis, neuronal loss and behavioral deficit in Cdk5 cKO mice. Interestingly, the dKO mice displayed a partial reversal in astrogliosis, but they still died at early age, suggesting that the increased expression of tPA in the cKO mice does not contribute significantly to the pathological process leading to neuroinflammation, neuronal loss and early lethality.Conclusion: The suppression of neuroinflammation in Cdk5 cKO mice ameliorates gliosis and neuronal loss, thus suggesting the potential beneficial effects of the PPARγ agonist pioglitazone for the treatment for neurodegenerative diseases.

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