Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway

Yukari Suzuki, Kazuhiro Ohgami, Kenji Shiratori, Xue Hai Jin, Iliyana Ilieva, Yoshikazu Koyama, Kazunaga Yazawa, Kazuhiko Yoshida, Satoru Kase, Shigeaki Ohno

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100 Citations (Scopus)

Abstract

We investigated the effects of astaxanthin (AST), a carotenoid, on endotoxin-induced uveitis (EIU), and over the course of the disease measured the expression of inflammatory cytokines and chemokines in the presence or absence of AST. EIU was induced in male Lewis rats by footpad injection of lipopolysaccharide (LPS). The animals were randomly divided to 12 groups with eight animals in each. Immediately after the inoculation, AST (1, 10, or 100 mg kg-1) was injected intravenously. Aqueous humour was collected at 6, 12 and 24 hr after LPS inoculation and the number of infiltrating cells in the anterior chamber was counted. In addition, we assayed the concentration of protein, nitric oxide (NO), tumour necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). Immunohistochemical staining with a monoclonal antibody against activated NF-κB was performed in order to evaluate the effects of AST on NF-κB activation. Rats injected with AST showed a significant decrease in the number of infiltrating cells in the anterior chamber and additionally there was a significantly lower concentration of protein, NO, TNF-α and PGE2 in the aqueous humour. Moreover, even early stages of EIU were suppressed by injection of AST. The number of activated NF-κB-positive cells was lower in iris-ciliary bodies treated with 10 or 100 mg kg-1 AST at 3 hr after LPS injection. These results suggest that AST reduces ocular inflammation in eyes with EIU by downregulating proinflammatory factors and by inhibiting the NF-κB-dependent signaling pathway.

Original languageEnglish
Pages (from-to)275-281
Number of pages7
JournalExperimental Eye Research
Volume82
Issue number2
DOIs
Publication statusPublished - 2006 Feb
Externally publishedYes

Keywords

  • Anti-inflammation agent
  • Endotoxin-induced uveitis
  • NF-κB

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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  • Cite this

    Suzuki, Y., Ohgami, K., Shiratori, K., Jin, X. H., Ilieva, I., Koyama, Y., Yazawa, K., Yoshida, K., Kase, S., & Ohno, S. (2006). Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway. Experimental Eye Research, 82(2), 275-281. https://doi.org/10.1016/j.exer.2005.06.023