Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway

Yukari Suzuki, Kazuhiro Ohgami, Kenji Shiratori, Xue Hai Jin, Iliyana Ilieva, Yoshikazu Koyama, Kazunaga Yazawa, Kazuhiko Yoshida, Satoru Kase, Shigeaki Ohno

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We investigated the effects of astaxanthin (AST), a carotenoid, on endotoxin-induced uveitis (EIU), and over the course of the disease measured the expression of inflammatory cytokines and chemokines in the presence or absence of AST. EIU was induced in male Lewis rats by footpad injection of lipopolysaccharide (LPS). The animals were randomly divided to 12 groups with eight animals in each. Immediately after the inoculation, AST (1, 10, or 100 mg kg-1) was injected intravenously. Aqueous humour was collected at 6, 12 and 24 hr after LPS inoculation and the number of infiltrating cells in the anterior chamber was counted. In addition, we assayed the concentration of protein, nitric oxide (NO), tumour necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). Immunohistochemical staining with a monoclonal antibody against activated NF-κB was performed in order to evaluate the effects of AST on NF-κB activation. Rats injected with AST showed a significant decrease in the number of infiltrating cells in the anterior chamber and additionally there was a significantly lower concentration of protein, NO, TNF-α and PGE2 in the aqueous humour. Moreover, even early stages of EIU were suppressed by injection of AST. The number of activated NF-κB-positive cells was lower in iris-ciliary bodies treated with 10 or 100 mg kg-1 AST at 3 hr after LPS injection. These results suggest that AST reduces ocular inflammation in eyes with EIU by downregulating proinflammatory factors and by inhibiting the NF-κB-dependent signaling pathway.

Original languageEnglish
Pages (from-to)275-281
Number of pages7
JournalExperimental Eye Research
Volume82
Issue number2
DOIs
Publication statusPublished - 2006 Feb
Externally publishedYes

Fingerprint

Uveitis
Endotoxins
Lipopolysaccharides
Aqueous Humor
Anterior Chamber
Dinoprostone
Injections
Nitric Oxide
Tumor Necrosis Factor-alpha
Cell Count
Ciliary Body
astaxanthine
Iris
Carotenoids
Chemokines
Proteins
Down-Regulation
Monoclonal Antibodies
Staining and Labeling
Cytokines

Keywords

  • Anti-inflammation agent
  • Endotoxin-induced uveitis
  • NF-κB

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway. / Suzuki, Yukari; Ohgami, Kazuhiro; Shiratori, Kenji; Jin, Xue Hai; Ilieva, Iliyana; Koyama, Yoshikazu; Yazawa, Kazunaga; Yoshida, Kazuhiko; Kase, Satoru; Ohno, Shigeaki.

In: Experimental Eye Research, Vol. 82, No. 2, 02.2006, p. 275-281.

Research output: Contribution to journalArticle

Suzuki, Y, Ohgami, K, Shiratori, K, Jin, XH, Ilieva, I, Koyama, Y, Yazawa, K, Yoshida, K, Kase, S & Ohno, S 2006, 'Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway', Experimental Eye Research, vol. 82, no. 2, pp. 275-281. https://doi.org/10.1016/j.exer.2005.06.023
Suzuki, Yukari ; Ohgami, Kazuhiro ; Shiratori, Kenji ; Jin, Xue Hai ; Ilieva, Iliyana ; Koyama, Yoshikazu ; Yazawa, Kazunaga ; Yoshida, Kazuhiko ; Kase, Satoru ; Ohno, Shigeaki. / Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway. In: Experimental Eye Research. 2006 ; Vol. 82, No. 2. pp. 275-281.
@article{b46d7ebb70ae45ba8a02c3d027a43a96,
title = "Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway",
abstract = "We investigated the effects of astaxanthin (AST), a carotenoid, on endotoxin-induced uveitis (EIU), and over the course of the disease measured the expression of inflammatory cytokines and chemokines in the presence or absence of AST. EIU was induced in male Lewis rats by footpad injection of lipopolysaccharide (LPS). The animals were randomly divided to 12 groups with eight animals in each. Immediately after the inoculation, AST (1, 10, or 100 mg kg-1) was injected intravenously. Aqueous humour was collected at 6, 12 and 24 hr after LPS inoculation and the number of infiltrating cells in the anterior chamber was counted. In addition, we assayed the concentration of protein, nitric oxide (NO), tumour necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). Immunohistochemical staining with a monoclonal antibody against activated NF-κB was performed in order to evaluate the effects of AST on NF-κB activation. Rats injected with AST showed a significant decrease in the number of infiltrating cells in the anterior chamber and additionally there was a significantly lower concentration of protein, NO, TNF-α and PGE2 in the aqueous humour. Moreover, even early stages of EIU were suppressed by injection of AST. The number of activated NF-κB-positive cells was lower in iris-ciliary bodies treated with 10 or 100 mg kg-1 AST at 3 hr after LPS injection. These results suggest that AST reduces ocular inflammation in eyes with EIU by downregulating proinflammatory factors and by inhibiting the NF-κB-dependent signaling pathway.",
keywords = "Anti-inflammation agent, Endotoxin-induced uveitis, NF-κB",
author = "Yukari Suzuki and Kazuhiro Ohgami and Kenji Shiratori and Jin, {Xue Hai} and Iliyana Ilieva and Yoshikazu Koyama and Kazunaga Yazawa and Kazuhiko Yoshida and Satoru Kase and Shigeaki Ohno",
year = "2006",
month = "2",
doi = "10.1016/j.exer.2005.06.023",
language = "English",
volume = "82",
pages = "275--281",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Suppressive effects of astaxanthin against rat endotoxin-induced uveitis by inhibiting the NF-κB signaling pathway

AU - Suzuki, Yukari

AU - Ohgami, Kazuhiro

AU - Shiratori, Kenji

AU - Jin, Xue Hai

AU - Ilieva, Iliyana

AU - Koyama, Yoshikazu

AU - Yazawa, Kazunaga

AU - Yoshida, Kazuhiko

AU - Kase, Satoru

AU - Ohno, Shigeaki

PY - 2006/2

Y1 - 2006/2

N2 - We investigated the effects of astaxanthin (AST), a carotenoid, on endotoxin-induced uveitis (EIU), and over the course of the disease measured the expression of inflammatory cytokines and chemokines in the presence or absence of AST. EIU was induced in male Lewis rats by footpad injection of lipopolysaccharide (LPS). The animals were randomly divided to 12 groups with eight animals in each. Immediately after the inoculation, AST (1, 10, or 100 mg kg-1) was injected intravenously. Aqueous humour was collected at 6, 12 and 24 hr after LPS inoculation and the number of infiltrating cells in the anterior chamber was counted. In addition, we assayed the concentration of protein, nitric oxide (NO), tumour necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). Immunohistochemical staining with a monoclonal antibody against activated NF-κB was performed in order to evaluate the effects of AST on NF-κB activation. Rats injected with AST showed a significant decrease in the number of infiltrating cells in the anterior chamber and additionally there was a significantly lower concentration of protein, NO, TNF-α and PGE2 in the aqueous humour. Moreover, even early stages of EIU were suppressed by injection of AST. The number of activated NF-κB-positive cells was lower in iris-ciliary bodies treated with 10 or 100 mg kg-1 AST at 3 hr after LPS injection. These results suggest that AST reduces ocular inflammation in eyes with EIU by downregulating proinflammatory factors and by inhibiting the NF-κB-dependent signaling pathway.

AB - We investigated the effects of astaxanthin (AST), a carotenoid, on endotoxin-induced uveitis (EIU), and over the course of the disease measured the expression of inflammatory cytokines and chemokines in the presence or absence of AST. EIU was induced in male Lewis rats by footpad injection of lipopolysaccharide (LPS). The animals were randomly divided to 12 groups with eight animals in each. Immediately after the inoculation, AST (1, 10, or 100 mg kg-1) was injected intravenously. Aqueous humour was collected at 6, 12 and 24 hr after LPS inoculation and the number of infiltrating cells in the anterior chamber was counted. In addition, we assayed the concentration of protein, nitric oxide (NO), tumour necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). Immunohistochemical staining with a monoclonal antibody against activated NF-κB was performed in order to evaluate the effects of AST on NF-κB activation. Rats injected with AST showed a significant decrease in the number of infiltrating cells in the anterior chamber and additionally there was a significantly lower concentration of protein, NO, TNF-α and PGE2 in the aqueous humour. Moreover, even early stages of EIU were suppressed by injection of AST. The number of activated NF-κB-positive cells was lower in iris-ciliary bodies treated with 10 or 100 mg kg-1 AST at 3 hr after LPS injection. These results suggest that AST reduces ocular inflammation in eyes with EIU by downregulating proinflammatory factors and by inhibiting the NF-κB-dependent signaling pathway.

KW - Anti-inflammation agent

KW - Endotoxin-induced uveitis

KW - NF-κB

UR - http://www.scopus.com/inward/record.url?scp=31044445848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31044445848&partnerID=8YFLogxK

U2 - 10.1016/j.exer.2005.06.023

DO - 10.1016/j.exer.2005.06.023

M3 - Article

VL - 82

SP - 275

EP - 281

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 2

ER -