Suppressive effects of the leaf of Terminalia catappa L. on osteoclast differentiation in vitro and bone weight loss in vivo

Tomoyuki Koyama, Chie Nakajima, Sogo Nishimoto, Masamichi Takami, Je Tae Woo, Kazunaga Yazawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Oral administration of Terminalia catappa extract (TCE; 1,000 mg/kg) for 5 wk suppressed bone weight loss and trabecular bone loss in ovariectomized mice. An in vitro experiment showed that TCE (1.3-20 μ g/mL) did not increase alkaline phosphatase activity, which would indicate osteoclast formation, in osteoblast-like 3T3-L1 cells. On the other hand, TCE (12.5 μ g/mL) markedly decreased the number of tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells, which would indicate osteoclast formation, in a co-culture system (bone marrow cells/osteoblastic UAMS-32 cells). A detailed analysis of the stages of osteoclast differentiation revealed that TCE mainly suppressed the differentiation of bone marrow mononuclear cells into osteoclast progenitor cells in the presence of MCSF and TGF- β. An additional experiment using fractionated TCE revealed that the watersoluble fraction suppressed the bone weight loss in OVX-mice and osteoclast differentiation in vitro. Therefore, the suppressive effects of TCE on bone weight loss in mice might be due to the suppressive effects of highly polar components on the early stage of osteoclast differentiation.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalJournal of Nutritional Science and Vitaminology
Volume58
Issue number2
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Terminalia
Osteoclasts
Weight Loss
Bone and Bones
Bone Marrow Cells
3T3-L1 Cells
Coculture Techniques
Osteoblasts
Alkaline Phosphatase
Oral Administration
In Vitro Techniques
Stem Cells

Keywords

  • Bone weight loss
  • Leaf of Terminalia catappa
  • Osteoclast differentiation
  • Prevention of osteoporosis
  • Variectomized mice

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Suppressive effects of the leaf of Terminalia catappa L. on osteoclast differentiation in vitro and bone weight loss in vivo. / Koyama, Tomoyuki; Nakajima, Chie; Nishimoto, Sogo; Takami, Masamichi; Woo, Je Tae; Yazawa, Kazunaga.

In: Journal of Nutritional Science and Vitaminology, Vol. 58, No. 2, 2012, p. 129-135.

Research output: Contribution to journalArticle

Koyama, Tomoyuki ; Nakajima, Chie ; Nishimoto, Sogo ; Takami, Masamichi ; Woo, Je Tae ; Yazawa, Kazunaga. / Suppressive effects of the leaf of Terminalia catappa L. on osteoclast differentiation in vitro and bone weight loss in vivo. In: Journal of Nutritional Science and Vitaminology. 2012 ; Vol. 58, No. 2. pp. 129-135.
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