Supramolecular ultrathin film strategies for DNA assemblies: Substrates for optobioelectronics, gene therapy, and microarrays

Rigoberto C. Advincula, Yingfan Wang, Mi Kyoung Park, Gautam Bhatia, Seth Steplelon, Cara Monroe, Iman Shelton, Wally Blanton, Xiaowu Fan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We describe our strategies and results in the preparation of supramolecularly ordered ultrathin films of DNA assemblies using the layer-by-layer (LbL) alternate polyelectrolyte adsorption technique. The properties of DNA are intimately associated with their polyelectrolyte behavior in solution. Deposition at interfaces is governed by conformation, orientation, and charge density of these biomolecules in relation to the physisorption phenomena in oppositely charged surfaces. Thus, controlling the nature of surfaces (polymer charge density, ionic strength, other non-covalent interactions, elc.) is important in modifying the adsorption phenomena. In this work, differences in adsorption and incorporation of DNA with dyes, linear polymers and dendrimers are highlighted. A number of surface sensitive spectroscopic and microscopic techniques were used to probe the adsorption and multilayer assembly phenomena, e.g. surface plasmon resonance spectroscopy (SPS), AFM, quartz crystal microbalance (QCM) and ellipsometry. These studies are important for future applications such as the use of polycations as non-viral gene transfection vectors for drug-delivery and DNA adsorption on microarray surfaces. By combining with the alternate assembly of azobenzene and phthalocyanine dyes, we have been able to prepare optobiolelectronic substrates where the phenomena of irradiation and electrochemistry can be used to probe the ordering and response of these films.

Original languageEnglish
Title of host publicationMolecular and Biomolecular Electronics
Pages55-60
Number of pages6
Volume679
Publication statusPublished - 2001
Externally publishedYes
Event2001 MRS Spring Meeting - San Francisco, CA
Duration: 2001 Apr 162001 Apr 20

Other

Other2001 MRS Spring Meeting
CitySan Francisco, CA
Period01/4/1601/4/20

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

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    Advincula, R. C., Wang, Y., Park, M. K., Bhatia, G., Steplelon, S., Monroe, C., Shelton, I., Blanton, W., & Fan, X. (2001). Supramolecular ultrathin film strategies for DNA assemblies: Substrates for optobioelectronics, gene therapy, and microarrays. In Molecular and Biomolecular Electronics (Vol. 679, pp. 55-60)