Sustainable antimicrobial effect of silver sulfadiazine-loaded nanosheets on infection in a mouse model of partial-thickness burn injury

Keisuke Ito, Akihiro Saito, Toshinori Fujie, Keisuke Nishiwaki, Hiromi Miyazaki, Manabu Kinoshita, Daizoh Saitoh, Shinya Ohtsubo, Shinji Takeoka

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Partial-thickness burn injury has the potential for reepithelialization and heals within 3 weeks. If the wound is infected by bacteria before reepithelization, however, the depth of disruption increases and the lesion easily progresses to the full-thickness dermal layers. In the treatment of partial-thickness burn injury, it is important to prevent the wound area from bacterial infection with an antimicrobial dressing. Here, we have tested the antimicrobial properties of polymeric ultra-thin films composed of poly(lactic acid) (termed "PLA nanosheets"), which have high flexibility, adhesive strength and transparency, and silver sulfadiazine (AgSD), which exhibits antimicrobial efficacy. The AgSD-loaded nanosheet released Ag<sup>+</sup> for more than 3 days, and exerted antimicrobial efficacy against methicillin-resistant Staphylococcus aureus (MRSA) in an in vitro Kirby-Bauer test. By contrast, a cell viability assay indicated that the dose of AgSD used in the PLA nanosheets did not show significant cytotoxicity toward fibroblasts. In vivo evaluation using a mouse model of infection in a partial-thickness burn wound demonstrated that the nanosheet significantly reduced the number of MRSA bacteria on the lesion (more than 10<sup>5</sup>-fold) and suppressed the inflammatory reaction, thereby preventing a protracted wound healing process.

Original languageEnglish
Article number3734
Pages (from-to)87-95
Number of pages9
JournalActa Biomaterialia
Volume24
DOIs
Publication statusPublished - 2015 Sep 15

Keywords

  • Antimicrobial
  • Poly(lactic acid)
  • Polymer nanosheet
  • Silver sulfadiazine
  • Wound dressing

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

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