Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior

Claudio G. Rolli, Hidekazu Nakayama, Kazuo Yamaguchi, Joachim P. Spatz, Ralf Kemkemer, Jun Nakanishi

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Collective cell migration plays a major role in cancer metastasis and wound healing, therefore, several invitro assays for studying such behavior have been developed. Using photoswitchable surfaces, we studied collective cell expansion behavior from initially precisely controlled adhesive patterns. A non-adhesive poly(ethylene glycol) (PEG) layer is conjugated to a glass coverslip via 2-nitrobenzyl groups, which cleave upon exposure to UV light, changing the surface from non-cell-adhesive to cell-adhesive without mechanical interference. Initial cell attaching areas are generated in arbitrary shapes via projection exposure through a photomask. After a growth phase, epithelial cell sheets are released from their confinement by a second illumination allowing for collective cell expansion. Our experiments with epithelial cells show that cluster size and boundary curvature modulate the expansion of the cell sheet and the formation of leader cells. At a certain cluster size, characteristics of the expansion behavior change and cells in the core are hardly affected by the boundary release. With donut-like ring structures, we demonstrate a break in symmetry between the behavior of cells along the outer convex boundary and along the inner concave boundary. Additionally, we observe that collective migration characteristics are modulated by the initial incubation time of the cell sheet.

Original languageEnglish
Pages (from-to)2409-2418
Number of pages10
JournalBiomaterials
Volume33
Issue number8
DOIs
Publication statusPublished - 2012 Mar 1
Externally publishedYes

Fingerprint

Adhesives
Geometry
Substrates
Polyethylene glycols
Photomasks
Ultraviolet radiation
Assays
Lighting
Cells
Epithelial Cells
Glass
Ethylene Glycol
Ultraviolet Rays
Wound Healing
Cell Movement
Experiments
Neoplasm Metastasis
Growth

Keywords

  • Cell adhesion
  • Cell spreading
  • Collective cell migration
  • Passivation
  • Photopatterning
  • Wound healing

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Rolli, C. G., Nakayama, H., Yamaguchi, K., Spatz, J. P., Kemkemer, R., & Nakanishi, J. (2012). Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior. Biomaterials, 33(8), 2409-2418. https://doi.org/10.1016/j.biomaterials.2011.12.012

Switchable adhesive substrates : Revealing geometry dependence in collective cell behavior. / Rolli, Claudio G.; Nakayama, Hidekazu; Yamaguchi, Kazuo; Spatz, Joachim P.; Kemkemer, Ralf; Nakanishi, Jun.

In: Biomaterials, Vol. 33, No. 8, 01.03.2012, p. 2409-2418.

Research output: Contribution to journalArticle

Rolli, CG, Nakayama, H, Yamaguchi, K, Spatz, JP, Kemkemer, R & Nakanishi, J 2012, 'Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior', Biomaterials, vol. 33, no. 8, pp. 2409-2418. https://doi.org/10.1016/j.biomaterials.2011.12.012
Rolli CG, Nakayama H, Yamaguchi K, Spatz JP, Kemkemer R, Nakanishi J. Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior. Biomaterials. 2012 Mar 1;33(8):2409-2418. https://doi.org/10.1016/j.biomaterials.2011.12.012
Rolli, Claudio G. ; Nakayama, Hidekazu ; Yamaguchi, Kazuo ; Spatz, Joachim P. ; Kemkemer, Ralf ; Nakanishi, Jun. / Switchable adhesive substrates : Revealing geometry dependence in collective cell behavior. In: Biomaterials. 2012 ; Vol. 33, No. 8. pp. 2409-2418.
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