Synchronised expressions of LPXRFamide peptide and its receptor genes: Seasonal, diurnal and circadian changes during spawning period in grass puffer

M. Shahjahan, T. Ikegami, T. Osugi, K. Ukena, H. Doi, A. Hattori, Kazuyoshi Tsutsui, H. Ando

    Research output: Contribution to journalArticle

    62 Citations (Scopus)

    Abstract

    Among the RFamide peptide family, the LPXRFamide peptide (LPXRFa) group regulates the release of various pituitary hormones and, recently, LPXRFa genes were found to be regulated by photoperiod via melatonin. As a first step towards investigating the role of LPXRFa on reproductive function in grass puffer (Takifugu niphobles), which spawns in semilunar cycles, genes encoding LPXRFa and its receptor (LPXRFa-R) were cloned, and seasonal, diurnal and circadian changes in their absolute amounts of mRNAs in the brain and pituitary were examined by quantitative real-time polymerase chain reaction. The grass puffer LPXRFa precursor contains two putative RFamide peptides and one possible RYamide peptide. LPXRFa and LPXRFa-R genes were extensively expressed in the diencephalon and pituitary. The expression levels of both genes were significantly elevated during the spawning periods in both sexes in the brain and pituitary, although they were low in the spawning fish just after releasing eggs and sperm. The treatment of primary pituitary cultures with goldfish LPXRFa increased the amounts of follicle-stimulating hormone β- and luteinising hormone β-subunit mRNAs. In the diencephalon, LPXRFa and LPXRFa-R genes showed synchronised diurnal and circadian variations with one peak at zeitgeber time 3 and circadian time 15, respectively. The correlated expression patterns of LPXRFa and LPXRFa-R genes in the diencephalon and pituitary and the possible stimulatory effects of LPXRFa on gonadotrophin subunit gene expression suggest the functional significance of the LPXRFa and LPXRFa-R system in the regulation of lunar-synchronised spawning of grass puffer.

    Original languageEnglish
    Pages (from-to)39-51
    Number of pages13
    JournalJournal of Neuroendocrinology
    Volume23
    Issue number1
    DOIs
    Publication statusPublished - 2011 Jan

    Fingerprint

    Tetraodontiformes
    Peptide Receptors
    Poaceae
    Peptides
    Genes
    vpr Genes
    Diencephalon
    Takifugu
    Messenger RNA
    Goldfish
    Pituitary Hormones
    Photoperiod
    Brain
    Follicle Stimulating Hormone
    Melatonin
    Luteinizing Hormone
    Gonadotropins

    Keywords

    • Circadian rhythm
    • GnIH
    • LPXRFamide peptide
    • RFamide-related peptide
    • Seasonal reproduction

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism
    • Endocrine and Autonomic Systems
    • Cellular and Molecular Neuroscience

    Cite this

    Synchronised expressions of LPXRFamide peptide and its receptor genes : Seasonal, diurnal and circadian changes during spawning period in grass puffer. / Shahjahan, M.; Ikegami, T.; Osugi, T.; Ukena, K.; Doi, H.; Hattori, A.; Tsutsui, Kazuyoshi; Ando, H.

    In: Journal of Neuroendocrinology, Vol. 23, No. 1, 01.2011, p. 39-51.

    Research output: Contribution to journalArticle

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    AU - Doi, H.

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