Synergistic Inhibition of Fructose 1,6‐Bisphosphatase by Fructose 2,6‐Bisphosphate and Adenosine Monophosphate in Round Spermatids of Rats

fructose 1, 6‐bisphosphatase/AMP/fructose 2, 6‐bisphosphate/spermatids/rats

MASAHISA NAKAMURA, AKIRA SUZUKI, SHOICHI OKINAGA, KIYOSHI ARAI

Research output: Contribution to journalArticle

Abstract

The effects of adenosine monophosphate (AMP) and fructose 2, 6‐bisphosphate (fruc‐2, 6‐P2) on the key‐enzyme of gluconeogenesis, fructose 1, 6‐bisphosphatase (fruc‐P2ase; D‐fructose 1, 6‐bisphosphate 1‐phosphohydrolase, EC 3.1.3.11) in spermatid extract from rat testes were studied. The fruc‐P2ase activity in the spermatids of rats was suppressed by AMP and fruc‐2, 6‐P2. The inhibition of fruc‐2, 6‐P2 was much stronger at low than at high substrate concentrations, and enhanced synergistically with AMP. The substrate saturation curve was changed by fruc‐2, 6‐P2 hyperbolic to sigmoidal. Furthermore, the concentration of AMP that decreased the activity to 50% was much lower in the presence than in the absence of fruc‐2, 6‐P2. These results indicate the possibility that gluconeogenesis in spermatids of rats is controlled by AMP and fruc‐2, 6‐P2.

Original languageEnglish
Pages (from-to)497-502
Number of pages6
JournalDevelopment Growth and Differentiation
Volume26
Issue number5
DOIs
Publication statusPublished - 1984
Externally publishedYes

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Spermatids
Adenosine Monophosphate
Fructose
Gluconeogenesis
Testis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

@article{2e39d53d642d4dc28e73f6d6109dbf91,
title = "Synergistic Inhibition of Fructose 1,6‐Bisphosphatase by Fructose 2,6‐Bisphosphate and Adenosine Monophosphate in Round Spermatids of Rats: fructose 1, 6‐bisphosphatase/AMP/fructose 2, 6‐bisphosphate/spermatids/rats",
abstract = "The effects of adenosine monophosphate (AMP) and fructose 2, 6‐bisphosphate (fruc‐2, 6‐P2) on the key‐enzyme of gluconeogenesis, fructose 1, 6‐bisphosphatase (fruc‐P2ase; D‐fructose 1, 6‐bisphosphate 1‐phosphohydrolase, EC 3.1.3.11) in spermatid extract from rat testes were studied. The fruc‐P2ase activity in the spermatids of rats was suppressed by AMP and fruc‐2, 6‐P2. The inhibition of fruc‐2, 6‐P2 was much stronger at low than at high substrate concentrations, and enhanced synergistically with AMP. The substrate saturation curve was changed by fruc‐2, 6‐P2 hyperbolic to sigmoidal. Furthermore, the concentration of AMP that decreased the activity to 50{\%} was much lower in the presence than in the absence of fruc‐2, 6‐P2. These results indicate the possibility that gluconeogenesis in spermatids of rats is controlled by AMP and fruc‐2, 6‐P2.",
author = "MASAHISA NAKAMURA and AKIRA SUZUKI and SHOICHI OKINAGA and KIYOSHI ARAI",
year = "1984",
doi = "10.1111/j.1440-169X.1984.00497.x",
language = "English",
volume = "26",
pages = "497--502",
journal = "Development Growth and Differentiation",
issn = "0012-1592",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Synergistic Inhibition of Fructose 1,6‐Bisphosphatase by Fructose 2,6‐Bisphosphate and Adenosine Monophosphate in Round Spermatids of Rats

T2 - fructose 1, 6‐bisphosphatase/AMP/fructose 2, 6‐bisphosphate/spermatids/rats

AU - NAKAMURA, MASAHISA

AU - SUZUKI, AKIRA

AU - OKINAGA, SHOICHI

AU - ARAI, KIYOSHI

PY - 1984

Y1 - 1984

N2 - The effects of adenosine monophosphate (AMP) and fructose 2, 6‐bisphosphate (fruc‐2, 6‐P2) on the key‐enzyme of gluconeogenesis, fructose 1, 6‐bisphosphatase (fruc‐P2ase; D‐fructose 1, 6‐bisphosphate 1‐phosphohydrolase, EC 3.1.3.11) in spermatid extract from rat testes were studied. The fruc‐P2ase activity in the spermatids of rats was suppressed by AMP and fruc‐2, 6‐P2. The inhibition of fruc‐2, 6‐P2 was much stronger at low than at high substrate concentrations, and enhanced synergistically with AMP. The substrate saturation curve was changed by fruc‐2, 6‐P2 hyperbolic to sigmoidal. Furthermore, the concentration of AMP that decreased the activity to 50% was much lower in the presence than in the absence of fruc‐2, 6‐P2. These results indicate the possibility that gluconeogenesis in spermatids of rats is controlled by AMP and fruc‐2, 6‐P2.

AB - The effects of adenosine monophosphate (AMP) and fructose 2, 6‐bisphosphate (fruc‐2, 6‐P2) on the key‐enzyme of gluconeogenesis, fructose 1, 6‐bisphosphatase (fruc‐P2ase; D‐fructose 1, 6‐bisphosphate 1‐phosphohydrolase, EC 3.1.3.11) in spermatid extract from rat testes were studied. The fruc‐P2ase activity in the spermatids of rats was suppressed by AMP and fruc‐2, 6‐P2. The inhibition of fruc‐2, 6‐P2 was much stronger at low than at high substrate concentrations, and enhanced synergistically with AMP. The substrate saturation curve was changed by fruc‐2, 6‐P2 hyperbolic to sigmoidal. Furthermore, the concentration of AMP that decreased the activity to 50% was much lower in the presence than in the absence of fruc‐2, 6‐P2. These results indicate the possibility that gluconeogenesis in spermatids of rats is controlled by AMP and fruc‐2, 6‐P2.

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U2 - 10.1111/j.1440-169X.1984.00497.x

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