Synthesis and characterization of artificial red cell (ARC)

Eishun Tsuchida, Hiroyuki Nishide

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Liposome-based red cell substitutes are very interesting because of their similarity in structure to red blood cells. Liposomes encapsulate not only Hb but also various regulators, so that they are able to share multiple functions like red blood cells. The author has succeeded in synthesizing such artificial cells by encapsulating an Hb solution with polylipid (stabilized bilayer membrane). This paper describes the synthesis, characterization and oxygen-transporting ability of this polylipid-based artificial red cell (ARC). Polylipid-encapsulated Hb was prepared by encapsulation of COHb under mild conditions, polymerization and decarbonylation. The average diameter was 0.2 μm and the distribution was sharp. The oxygen-transporting ability in vitro was as follows: P50: 30-40 mmHg, OTE: 40%, n: approximately 2, [Hb]: approximately 10 g/dl, metHb: <5%. The viscosity was low (approximately 8 cP at 37°C) and the low colloidal osmotic pressure (0.1 mmHg/g/dl) could be adjusted to normal by adding human albumin. Polylipid/Hb microparticles were physically stable and the suspension could be frozen. After thawing, no changes in physical properties and oxygen-transporting ability were noted and no leakage of Hb. The product could be stored in the frozen state longer than six months without any change in quality. Polylipid/Hb is an artificial red cell based on stable liposomes, and its excellent properties demonstrate its high potential as a red cell substitute.

Original languageEnglish
Pages (from-to)494
Number of pages1
JournalBiomaterials, Artificial Cells, and Immobilization Biotechnology
Volume19
Issue number2
Publication statusPublished - 1991

Fingerprint

Artificial Cells
Liposomes
Blood Substitutes
Cells
Oxygen
Erythrocytes
Osmotic Pressure
Viscosity
Polymerization
Albumins
Suspensions
Blood
Thawing
Membranes
Encapsulation
Physical properties

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Synthesis and characterization of artificial red cell (ARC). / Tsuchida, Eishun; Nishide, Hiroyuki.

In: Biomaterials, Artificial Cells, and Immobilization Biotechnology, Vol. 19, No. 2, 1991, p. 494.

Research output: Contribution to journalArticle

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