Synthesis and characterization of poly(ethylene glycol)-glutathione conjugate self-assembled nanoparticles for antioxidant delivery

Sharlene R. Williams, Benjamin S. Lepene, Craig D. Thatcher, Timothy Edward Long

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Functional oligomers based on glutathione (GSH) and poly(ethylene glycol) diacrylate (PEGDA) were synthesized via Michael addition. Well-defined, spherical nanoparticle self-assembly was confirmed via dynamic light scattering and transmission electron microscopy. In addition, a series of Michael addition oligomers containing GSH were prepared with various molecular weights of poly(ethylene glycol) (PEG). Thermal analysis indicated that the oligomers were thermally stable to ∼160 °C, and the T g increased as the PEG molecular weight increased. In addition, thiol-terminated PEG was synthesized and reacted with GSH to form disulfide-linked oligomers to probe potential antioxidant therapies. SH-SY5Y cells were utilized in cell culture experiments, and hydrogen peroxide induced oxidative stress on the cells. Disulfide-linked GSH oligomers were 100% effective at protecting SH-SY5Y cells from oxidative stress, whereas the Michael addition GSH oligomers did not offer protection.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalBiomacromolecules
Volume10
Issue number1
DOIs
Publication statusPublished - 2009 Jan 12
Externally publishedYes

Fingerprint

Antioxidants
Oligomers
Polyethylene glycols
Nanoparticles
Oxidative stress
Disulfides
Molecular weight
Dynamic light scattering
Light transmission
Cell culture
Sulfhydryl Compounds
Hydrogen peroxide
Self assembly
Thermoanalysis
Hydrogen Peroxide
Glutathione
poly(ethylene glycol)-glutathione conjugate
Transmission electron microscopy
Experiments

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Synthesis and characterization of poly(ethylene glycol)-glutathione conjugate self-assembled nanoparticles for antioxidant delivery. / Williams, Sharlene R.; Lepene, Benjamin S.; Thatcher, Craig D.; Long, Timothy Edward.

In: Biomacromolecules, Vol. 10, No. 1, 12.01.2009, p. 155-161.

Research output: Contribution to journalArticle

Williams, Sharlene R. ; Lepene, Benjamin S. ; Thatcher, Craig D. ; Long, Timothy Edward. / Synthesis and characterization of poly(ethylene glycol)-glutathione conjugate self-assembled nanoparticles for antioxidant delivery. In: Biomacromolecules. 2009 ; Vol. 10, No. 1. pp. 155-161.
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