Synthesis and storage of prolactin in the pituitary gland of bullfrog tadpoles during metamorphosis

Kazutoshi Yamamoto, Kazuhiko Niinuma, Sakaé Kikuyama

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Changes in prolactin (PRL) levels and PRL-producing activities in the pituitary gland of tadpoles during metamorphosis were studied. PRL concentration in the pituitary gland as measured by a homologous radioimmunoassay was relatively low during pre- and prometamorphosis and early climax (2.5-3.5 μg/100 μg protein). PRL levels increased thereafter and reached maximum (7.3 μg/100 μg protein) at late climax (stage 24). It was also demon-strated that the pituitary cells which immunologically reacted with the antiserum to bullfrog PRL increased in number as metamorphosis proceeded. Measurement of incorporation of [3H]leucine into pituitary PRL in vitro revealed that elevation of PRL synthesis took place in the tadpoles which had reached climax. Radioimmunoassay and bioassay of PRL in the pituitary glands of adult frogs and tadpoles indicated that there was no difference in immunological and biological properties between adults and larvae.

    Original languageEnglish
    Pages (from-to)247-253
    Number of pages7
    JournalGeneral and Comparative Endocrinology
    Volume62
    Issue number2
    DOIs
    Publication statusPublished - 1986

    Fingerprint

    Rana catesbeiana
    pituitary gland
    Pituitary Gland
    tadpoles
    prolactin
    Prolactin
    metamorphosis
    Larva
    synthesis
    radioimmunoassays
    Radioimmunoassay
    Leucine
    Biological Assay
    Anura
    leucine
    antiserum
    Immune Sera
    frogs
    Proteins
    proteins

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Synthesis and storage of prolactin in the pituitary gland of bullfrog tadpoles during metamorphosis. / Yamamoto, Kazutoshi; Niinuma, Kazuhiko; Kikuyama, Sakaé.

    In: General and Comparative Endocrinology, Vol. 62, No. 2, 1986, p. 247-253.

    Research output: Contribution to journalArticle

    Yamamoto, Kazutoshi ; Niinuma, Kazuhiko ; Kikuyama, Sakaé. / Synthesis and storage of prolactin in the pituitary gland of bullfrog tadpoles during metamorphosis. In: General and Comparative Endocrinology. 1986 ; Vol. 62, No. 2. pp. 247-253.
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