TY - JOUR
T1 - Synthesis of heterotrimeric collagen models containing Arg residues in Y-positions and analysis of their conformational stability
AU - Koide, Takaki
AU - Nishikawa, Yoshimi
AU - Takahara, Yoshifumi
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (No. 15032238) by the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Dr. Akira Otaka for ESI-MS measurements. We also thank Dr. Kazuhiro Nagata and Mr. Norihisa Yasui for valuable discussion.
PY - 2004/1/5
Y1 - 2004/1/5
N2 - An Arg residue incorporated into the Y-position of collagenous host-guest peptide Ac-(Gly-Pro-Hyp)3-Gly-Pro-Y-(Gly-Pro-Hyp)4-Gly- Gly-NH2 is reported to stabilize the triple helical structure as well as a 4(R)-hydroxyproline (Hyp) residue. Here, we synthesized heterotrimeric collagen models containing Arg in Y-positions utilizing the cystine knot strategy. Analysis of their thermal transition temperatures using circular dichroism spectrometry demonstrated unexpected decrease in the triple helical stability as the number of Arg increased. The obtained results indicated that an Arg residue in a Y-position is not always an equivalent of a Hyp residue, and that it possesses a potential helix destabilizing effect.
AB - An Arg residue incorporated into the Y-position of collagenous host-guest peptide Ac-(Gly-Pro-Hyp)3-Gly-Pro-Y-(Gly-Pro-Hyp)4-Gly- Gly-NH2 is reported to stabilize the triple helical structure as well as a 4(R)-hydroxyproline (Hyp) residue. Here, we synthesized heterotrimeric collagen models containing Arg in Y-positions utilizing the cystine knot strategy. Analysis of their thermal transition temperatures using circular dichroism spectrometry demonstrated unexpected decrease in the triple helical stability as the number of Arg increased. The obtained results indicated that an Arg residue in a Y-position is not always an equivalent of a Hyp residue, and that it possesses a potential helix destabilizing effect.
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U2 - 10.1016/j.bmcl.2003.10.005
DO - 10.1016/j.bmcl.2003.10.005
M3 - Article
C2 - 14684312
AN - SCOPUS:0346887170
SN - 0960-894X
VL - 14
SP - 125
EP - 128
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 1
ER -