Synthesis of multiacyl poly(ethylene glycol) for the conjugation of cytochrome c to phospholipid vesicle

H. Ohkawa, Y. Teramura, Shinji Takeoka, E. Tsuchida

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    To conjugate water-soluble macromolecules on the surface of phospholipid vesicles, we synthesized a poly(ethylene glycol) (PEG)-lipid having four acyl chains using a lysine (Lys)-type monodendron structure. One end of the diamino-PEG was amidified with Lys, and then two amino groups of the Lys moiety were amidified with two Lys derivatives which had been acylated with two stearoyl groups. The other end of the PEG was activated with a triazine group or a pyridyldithio group. The hydrate of the lipid mixture of dipalmitoylphosphatidylcholine, cholesterol, dipalmitoylphosphatidylglycerol, and the PEG-lipid at a molar ratio of 5/5/1/0.3 was extruded in order to prepare the phospholipid vesicles with the average diameter of 270 ± 20 nm. The coupling ratio of cytochrome c with the PEG-lipid was monitored by HPLC, detecting the pyridyl 2-thione liberated from the pyridyldithio group and determining it to be 26% on the basis of the incorporated PEG-lipid.

    Original languageEnglish
    Pages (from-to)815-821
    Number of pages7
    JournalBioconjugate Chemistry
    Volume11
    Issue number6
    DOIs
    Publication statusPublished - 2000

    Fingerprint

    Ethylene Glycol
    Phospholipids
    Cytochromes c
    Polyethylene glycols
    Lysine
    Proteins
    Lipids
    Thiones
    1,2-Dipalmitoylphosphatidylcholine
    Triazines
    Cholesterol
    High Pressure Liquid Chromatography
    Hydrates
    Macromolecules
    Water
    Derivatives

    ASJC Scopus subject areas

    • Chemistry(all)
    • Organic Chemistry
    • Clinical Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry

    Cite this

    Synthesis of multiacyl poly(ethylene glycol) for the conjugation of cytochrome c to phospholipid vesicle. / Ohkawa, H.; Teramura, Y.; Takeoka, Shinji; Tsuchida, E.

    In: Bioconjugate Chemistry, Vol. 11, No. 6, 2000, p. 815-821.

    Research output: Contribution to journalArticle

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    AU - Takeoka, Shinji

    AU - Tsuchida, E.

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    Y1 - 2000

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