TY - JOUR
T1 - Synthesis of Polymeric Reconstituted Hemoglobins as Superoxide Dismutase Mimics Designed for Long-term Circulation Maintenance
AU - Yuasa, Makoto
AU - Oyaizu, Kenichi
AU - Hayashi, Takamitsu
AU - Yamaguchi, Aritomo
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2005
Y1 - 2005
N2 - Superoxide dismutase (SOD), catalase and glutathione peroxidase catalize the elimination of reactive oxygen species (ROS) such as a superoxide anion radical (O2-·). But enzyme activity decreases in diseases in which ROS attacks DNA, lipid and blood vessels. Recent studies on SOD mimics indicate that manganese porphyrin (MnT2MePyP) express high SOD activity. But the half-life of MnT2MePyP in the blood is usually very short. In the present study, a novel reconstituted polymerized hemoglobin containing manganese porphyrins (MnT2MePyP-PolyHb) was synthesized. SOD activity of MnT2MePyP-PolyHb (IC50 = 1.1 μM, kcat = 7.3 × 106 M-1s-1) was found to be somewhat less than that of MnT2MePyP (IC50 = 0.23 μM, kcat = 60 × 106 M-1s-1). MnT2MePyP-PolyHb half-life in H2O2 (t1/2 = 1070 s) was found to be as much as four times that of MnT2MePyP (t1/2 = 250 s). MnT2MePyP-PolyHb may thus be a valid SOD model for effectively maintaining and prolonging blood circulation.
AB - Superoxide dismutase (SOD), catalase and glutathione peroxidase catalize the elimination of reactive oxygen species (ROS) such as a superoxide anion radical (O2-·). But enzyme activity decreases in diseases in which ROS attacks DNA, lipid and blood vessels. Recent studies on SOD mimics indicate that manganese porphyrin (MnT2MePyP) express high SOD activity. But the half-life of MnT2MePyP in the blood is usually very short. In the present study, a novel reconstituted polymerized hemoglobin containing manganese porphyrins (MnT2MePyP-PolyHb) was synthesized. SOD activity of MnT2MePyP-PolyHb (IC50 = 1.1 μM, kcat = 7.3 × 106 M-1s-1) was found to be somewhat less than that of MnT2MePyP (IC50 = 0.23 μM, kcat = 60 × 106 M-1s-1). MnT2MePyP-PolyHb half-life in H2O2 (t1/2 = 1070 s) was found to be as much as four times that of MnT2MePyP (t1/2 = 250 s). MnT2MePyP-PolyHb may thus be a valid SOD model for effectively maintaining and prolonging blood circulation.
KW - cationic metalloporphyrin
KW - circuration persistence
KW - polymerized hemoglobin
KW - reconstituted hemoglobin
KW - superoxide dismutase mimic
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U2 - 10.5650/jos.54.413
DO - 10.5650/jos.54.413
M3 - Article
AN - SCOPUS:44649201021
VL - 54
SP - 413
EP - 418
JO - Journal of Oleo Science
JF - Journal of Oleo Science
SN - 1345-8957
IS - 7
ER -