Systematic single base-pair substitution analysis of DNA binding by the cAMP receptor protein in cyanobacterium Synechocystis sp. PCC 6803

Katsumi Omagari, Hidehisa Yoshimura, Mitsunori Takano, Dongyun Hao, Masayuki Ohmori, Akinori Sarai, Akira Suyama

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The cAMP receptor protein SYCRP1 in cyanobacterium Synechocystis sp. PCC 6803 is a regulatory protein that binds to the consensus DNA sequence (5′-AAATGTGATCTAGATCACATTT-3′) for the cAMP receptor protein CRP in Escherichia coli. Here we examined the effects of systematic single base-pair substitutions at positions 4-8 (TGTGA) of the consensus sequence on the specific binding of SYCRP1. The consensus sequence exhibited the highest affinity, and the effects of base-pair substitutions at positions 5 and 7 were the most deleterious. The result is similar to that previously reported for CRP, whereas there were differences between SYCRP1 and CRP in the rank order of affinity for each substitution.

Original languageEnglish
Pages (from-to)55-58
Number of pages4
JournalFEBS Letters
Volume563
Issue number1-3
DOIs
Publication statusPublished - 2004 Apr 9

Keywords

  • Binding free energy change
  • CRP, cAMP receptor protein
  • Cyanobacterium
  • Protein-DNA interaction
  • Regulatory protein
  • SYCRP1, cAMP receptor protein in cyanobacterium Synechocystis sp. PCC6803
  • Systematic base-pair substitution
  • cAMP receptor protein

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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