TY - JOUR
T1 - Systematic single base-pair substitution analysis of DNA binding by the cAMP receptor protein in cyanobacterium Synechocystis sp. PCC 6803
AU - Omagari, Katsumi
AU - Yoshimura, Hidehisa
AU - Takano, Mitsunori
AU - Hao, Dongyun
AU - Ohmori, Masayuki
AU - Sarai, Akinori
AU - Suyama, Akira
N1 - Funding Information:
This work was supported in part by a grant-in-aid from The 21st Century COE (Center of Excellence) program (Research Center for Integrated Science) of the Ministry of Education, Culture, Sports, Science, and Technology, Japan.
PY - 2004/4/9
Y1 - 2004/4/9
N2 - The cAMP receptor protein SYCRP1 in cyanobacterium Synechocystis sp. PCC 6803 is a regulatory protein that binds to the consensus DNA sequence (5′-AAATGTGATCTAGATCACATTT-3′) for the cAMP receptor protein CRP in Escherichia coli. Here we examined the effects of systematic single base-pair substitutions at positions 4-8 (TGTGA) of the consensus sequence on the specific binding of SYCRP1. The consensus sequence exhibited the highest affinity, and the effects of base-pair substitutions at positions 5 and 7 were the most deleterious. The result is similar to that previously reported for CRP, whereas there were differences between SYCRP1 and CRP in the rank order of affinity for each substitution.
AB - The cAMP receptor protein SYCRP1 in cyanobacterium Synechocystis sp. PCC 6803 is a regulatory protein that binds to the consensus DNA sequence (5′-AAATGTGATCTAGATCACATTT-3′) for the cAMP receptor protein CRP in Escherichia coli. Here we examined the effects of systematic single base-pair substitutions at positions 4-8 (TGTGA) of the consensus sequence on the specific binding of SYCRP1. The consensus sequence exhibited the highest affinity, and the effects of base-pair substitutions at positions 5 and 7 were the most deleterious. The result is similar to that previously reported for CRP, whereas there were differences between SYCRP1 and CRP in the rank order of affinity for each substitution.
KW - Binding free energy change
KW - CRP, cAMP receptor protein
KW - Cyanobacterium
KW - Protein-DNA interaction
KW - Regulatory protein
KW - SYCRP1, cAMP receptor protein in cyanobacterium Synechocystis sp. PCC6803
KW - Systematic base-pair substitution
KW - cAMP receptor protein
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U2 - 10.1016/S0014-5793(04)00248-0
DO - 10.1016/S0014-5793(04)00248-0
M3 - Article
C2 - 15063722
AN - SCOPUS:1842636916
SN - 0014-5793
VL - 563
SP - 55
EP - 58
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -