Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast

Ngang Heok Tang, Naoyuki Okada, Chii Shyang Fong, Kunio Arai, Masamitsu Sato, Takashi Toda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.

Original languageEnglish
Pages (from-to)2814-2821
Number of pages8
JournalFEBS Letters
Volume588
Issue number17
DOIs
Publication statusPublished - 2014 Aug 25

Keywords

  • Centrosome
  • Fission yeast
  • Pericentrin family
  • Spindle microtubule
  • Spindle pole body
  • TACC

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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