Telomerase activity during carcinogenesis in the bronchus

Masatoshi Kakihana, Naoyuki Yahata, Takashi Hirano, Hidetoshi Honda, Norihiko Ikeda, Norihiko Kawate, Chimori Konaka, Yoshiro Ebihara, Kazuma Ohyashiki, Harubumi Kato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Telomerase is a ribonucleoprotein reverse transcriptase that synthesizes telomeric DNA onto chromosome ends, and is not detected in most normal cells. It has been clarified that some bronchial squamous cell carcinomas may arise through the metaplasia and dysplasia sequence accompanied by accumulation of genetic mutations in metaplastic cells. Recently a highly sensitive polymerase chain reaction (PCR)-based telomerase assay (TRAP assay) was developed for the detection of telomerase activity. Telomerase activity has been found in most malignant neoplasms, including lung cancer. The objective of this study was to determine whether telomerase RNA might increase in precancerous lesions of the bronchi. Bronchial-brushing extracts were analyzed for telomerase activity (F-TRAP) and in situ telomerase activity using a fluorescence-based TRAP assay (in situ TRAP) and compared to cytological features. The fluorescence-based semi-quantitative TRAP assay detected telomerase activity in 8 out of 12 lung cancer cases (66.7%). In squamous cell carcinoma, 6 out of 9 cases (66.7%) showed telomerase activity. On the other hand, in normal and precancerous lesions of the bronchi, telomerase activity was not detected using either the F-TRAP method or in situ TRAP method. We concluded that dysplastic cells might not contain immortalized cells, and that the increase of telomerase activity is a relatively late event during the bronchial carcinogenesis. It is difficult to distinguish between dysplasia and in situ carcinoma of the bronchus morphologically, but the measurement of telomerase activity is clinically valuable for the determination of treatment.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalOncology Reports
Volume9
Issue number1
Publication statusPublished - 2002 Jan
Externally publishedYes

Fingerprint

Telomerase
Bronchi
Carcinogenesis
Squamous Cell Carcinoma
Lung Neoplasms
Fluorescence
Ribonucleoproteins
RNA-Directed DNA Polymerase
Carcinoma in Situ
Metaplasia
Chromosomes
Polymerase Chain Reaction

Keywords

  • In situ
  • Lung cancer
  • Squamous metaplasia
  • Telomerase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kakihana, M., Yahata, N., Hirano, T., Honda, H., Ikeda, N., Kawate, N., ... Kato, H. (2002). Telomerase activity during carcinogenesis in the bronchus. Oncology Reports, 9(1), 43-49.

Telomerase activity during carcinogenesis in the bronchus. / Kakihana, Masatoshi; Yahata, Naoyuki; Hirano, Takashi; Honda, Hidetoshi; Ikeda, Norihiko; Kawate, Norihiko; Konaka, Chimori; Ebihara, Yoshiro; Ohyashiki, Kazuma; Kato, Harubumi.

In: Oncology Reports, Vol. 9, No. 1, 01.2002, p. 43-49.

Research output: Contribution to journalArticle

Kakihana, M, Yahata, N, Hirano, T, Honda, H, Ikeda, N, Kawate, N, Konaka, C, Ebihara, Y, Ohyashiki, K & Kato, H 2002, 'Telomerase activity during carcinogenesis in the bronchus', Oncology Reports, vol. 9, no. 1, pp. 43-49.
Kakihana M, Yahata N, Hirano T, Honda H, Ikeda N, Kawate N et al. Telomerase activity during carcinogenesis in the bronchus. Oncology Reports. 2002 Jan;9(1):43-49.
Kakihana, Masatoshi ; Yahata, Naoyuki ; Hirano, Takashi ; Honda, Hidetoshi ; Ikeda, Norihiko ; Kawate, Norihiko ; Konaka, Chimori ; Ebihara, Yoshiro ; Ohyashiki, Kazuma ; Kato, Harubumi. / Telomerase activity during carcinogenesis in the bronchus. In: Oncology Reports. 2002 ; Vol. 9, No. 1. pp. 43-49.
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