Temperature dependence for anammox bacteria enriched from freshwater sediments

Toshifumi Osaka, Yuya Kimura, Yosuke Otsubo, Yuichi Suwa, Satoshi Tsuneda, Kazuichi Isaka

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    The anoxic ammonium oxidation (anammox) process has been regarded as an attractive alternative process to treat wastewater containing high ammonium concentrations. By the implementation of anammox process at moderately low temperatures (<25°C), the anammox process will be applied to more various industrial wastewater treatments. In this study, we established enrichment cultures of anammox bacteria from freshwater sediments by using an up-flow column reactor equipped with porous polyester nonwoven fabric at moderately low temperatures. Their nitrogen conversion rates reached 0.07-0.26kg-N/m3/d. Phylogenetic analysis based on 16S rRNA gene from enrichment cultures revealed the presence of various anammox bacteria affiliated with unknown anammox bacteria as well as known anammox candidates, i.e., Candidatus Kuenenia stuttgartiensis and Candidatus Brocadia fulgida, Candidatus Scalindua wagneri. Anammox bacterial populations were influenced by enrichment conditions, i.e., seed sediments and temperature.

    Original languageEnglish
    Pages (from-to)429-434
    Number of pages6
    JournalJournal of Bioscience and Bioengineering
    Volume114
    Issue number4
    DOIs
    Publication statusPublished - 2012 Oct

    Fingerprint

    Fresh Water
    Ammonium Compounds
    Bacteria
    Sediments
    Oxidation
    Temperature
    Waste Water
    Nonwoven fabrics
    Polyesters
    Cell culture
    Wastewater treatment
    Seed
    rRNA Genes
    Wastewater
    Genes
    Seeds
    Nitrogen
    Population

    Keywords

    • 16S rRNA phylogenic analysis
    • Anammox
    • Biological nitrogen cycle
    • Freshwater environment
    • Moderately low temperature

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Temperature dependence for anammox bacteria enriched from freshwater sediments. / Osaka, Toshifumi; Kimura, Yuya; Otsubo, Yosuke; Suwa, Yuichi; Tsuneda, Satoshi; Isaka, Kazuichi.

    In: Journal of Bioscience and Bioengineering, Vol. 114, No. 4, 10.2012, p. 429-434.

    Research output: Contribution to journalArticle

    Osaka, Toshifumi ; Kimura, Yuya ; Otsubo, Yosuke ; Suwa, Yuichi ; Tsuneda, Satoshi ; Isaka, Kazuichi. / Temperature dependence for anammox bacteria enriched from freshwater sediments. In: Journal of Bioscience and Bioengineering. 2012 ; Vol. 114, No. 4. pp. 429-434.
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