Temperature dependence of force, velocity, and processivity of single kinesin molecules

Kenji Kawaguchi, Shin'Ichi Ishiwata

    Research output: Contribution to journalArticle

    89 Citations (Scopus)

    Abstract

    Using the bead assay in optical microscopy equipped with optical tweezers, we have examined the effect of temperature on the gliding velocity, force, and processivity of single kinesin molecules interacting with a microtubule between 15 and 35°C. The gliding velocity increased with the Arrhenius activation energy of 50 kJ/mol, consistent with the temperature dependence of the microtubule-dependent ATPase activity. Also, the average run length, i.e., a measure of processivity of kinesin, increased on increasing temperature. On the other hand, the generated force was independent of temperature, 7.34 ± 0.33 pN (average ± S.D., n = 70). The gliding velocities decreased almost linearly with an increase in force irrespective of temperature, implying that the efficiency of mechanochemical energy conversion is maintained constant in this temperature range. Thus, we suggest that the force generation is attributable to the temperature-insensitive nucleotide-binding state(s) and/or conformational change(s) of kinesin-microtubule complex, whereas the gliding velocity is determined by the ATPase rate. (C) 2000 Academic Press.

    Original languageEnglish
    Pages (from-to)895-899
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume272
    Issue number3
    DOIs
    Publication statusPublished - 2000 Jun 16

    Fingerprint

    Kinesin
    Molecules
    Temperature
    Microtubules
    Adenosine Triphosphatases
    Optical Tweezers
    Optical tweezers
    Energy conversion
    Optical microscopy
    Microscopy
    Assays
    Nucleotides
    Activation energy

    Keywords

    • Arrhenius activation energy
    • Force generation
    • Gliding velocity
    • Kinesin
    • Microtubule
    • Motor proteins
    • Processivity
    • Single molecule analysis
    • Temperature effect

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Temperature dependence of force, velocity, and processivity of single kinesin molecules. / Kawaguchi, Kenji; Ishiwata, Shin'Ichi.

    In: Biochemical and Biophysical Research Communications, Vol. 272, No. 3, 16.06.2000, p. 895-899.

    Research output: Contribution to journalArticle

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