Temperature-induced precipitation of specific DNA fragments using DNA-poly (N-isopropylacrylamide) conjugate

Daisuke Umeno; Mizuo Maeda

doi:10.1246/cl.1999.381

Temperature-induced precipitation of specific DNA fragments using DNA-poly (N-isopropylacrylamide) conjugate

Daisuke Umeno, Mizuo Maeda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Soluble conjugates between double stranded DNA and poly(N-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.

Original language	English
Pages (from-to)	381-382
Number of pages	2
Journal	Chemistry Letters
Issue number	5
DOIs	https://doi.org/10.1246/cl.1999.381
Publication status	Published - 1999
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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10.1246/cl.1999.381

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title = "Temperature-induced precipitation of specific DNA fragments using DNA-poly (N-isopropylacrylamide) conjugate",

abstract = "Soluble conjugates between double stranded DNA and poly(N-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.",

author = "Daisuke Umeno and Mizuo Maeda",

year = "1999",

doi = "10.1246/cl.1999.381",

language = "English",

pages = "381--382",

journal = "Chemistry Letters",

issn = "0366-7022",

publisher = "Chemical Society of Japan",

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T1 - Temperature-induced precipitation of specific DNA fragments using DNA-poly (N-isopropylacrylamide) conjugate

AU - Umeno, Daisuke

AU - Maeda, Mizuo

PY - 1999

Y1 - 1999

N2 - Soluble conjugates between double stranded DNA and poly(N-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.

AB - Soluble conjugates between double stranded DNA and poly(N-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.

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U2 - 10.1246/cl.1999.381

DO - 10.1246/cl.1999.381

M3 - Article

AN - SCOPUS:0033241973

SN - 0366-7022

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EP - 382

JO - Chemistry Letters

JF - Chemistry Letters

IS - 5

ER -

Temperature-induced precipitation of specific DNA fragments using DNA-poly (N-isopropylacrylamide) conjugate

Abstract

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