Temperature-induced precipitation of specific DNA fragments using DNA-poly (N-isopropylacrylamide) conjugate

Daisuke Umeno, Mizuo Maeda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Soluble conjugates between double stranded DNA and poly(N-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.

Original languageEnglish
Pages (from-to)381-382
Number of pages2
JournalChemistry Letters
Issue number5
Publication statusPublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)


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