Template-dependent peptide formation on ribosomes catalyzed by pyridine

Itaru Nitta, Takuya Ueda, Kimitsuna Watanabe

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11 Citations (Scopus)

Abstract

We found that poly(U)-dependent polyphenylalanine synthesis on Escherichia coli ribosomes was extremely accelerated in the presence of a high concentration of pyridine. In this reaction, chemical energy sources, such as ATP and GTP, and soluble protein factors are not required, but the template and ribosomes are essential for the progress of the reaction. The reaction was inhibited by the antibiotics which inhibit the usual bacterial translation process on the ribosomes. These observations clearly demonstrate that the pyridine-catalyzed amino acid condensation reaction proceeds on the ribosome.

Original languageEnglish
Pages (from-to)803-807
Number of pages5
JournalJournal of biochemistry
Volume115
Issue number4
DOIs
Publication statusPublished - 1994 Apr

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Keywords

  • Aminoacyl-tRNA
  • Antibiotic
  • Pyridine
  • Ribosome
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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