Template-dependent peptide formation on ribosomes catalyzed by pyridine

Itaru Nitta, Takuya Ueda, Kimitsuna Watanabe

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We found that poly(U)-dependent polyphenylalanine synthesis on Escherichia coli ribosomes was extremely accelerated in the presence of a high concentration of pyridine. In this reaction, chemical energy sources, such as ATP and GTP, and soluble protein factors are not required, but the template and ribosomes are essential for the progress of the reaction. The reaction was inhibited by the antibiotics which inhibit the usual bacterial translation process on the ribosomes. These observations clearly demonstrate that the pyridine-catalyzed amino acid condensation reaction proceeds on the ribosome.

Original languageEnglish
Pages (from-to)803-807
Number of pages5
JournalJournal of biochemistry
Volume115
Issue number4
DOIs
Publication statusPublished - 1994 Jan 1
Externally publishedYes

Fingerprint

Ribosomes
Poly U
Peptides
Condensation reactions
Guanosine Triphosphate
Escherichia coli
Chemical reactions
Bacterial Physiological Phenomena
Adenosine Triphosphate
Anti-Bacterial Agents
Amino Acids
Proteins
pyridine
polyphenylalanine

Keywords

  • Aminoacyl-tRNA
  • Antibiotic
  • Pyridine
  • Ribosome
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Template-dependent peptide formation on ribosomes catalyzed by pyridine. / Nitta, Itaru; Ueda, Takuya; Watanabe, Kimitsuna.

In: Journal of biochemistry, Vol. 115, No. 4, 01.01.1994, p. 803-807.

Research output: Contribution to journalArticle

Nitta, Itaru ; Ueda, Takuya ; Watanabe, Kimitsuna. / Template-dependent peptide formation on ribosomes catalyzed by pyridine. In: Journal of biochemistry. 1994 ; Vol. 115, No. 4. pp. 803-807.
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