Temporal fluctuation in the abundance of alginate-degrading bacteria in the gut of abalone Haliotis gigantea over 1 year

Reiji Tanaka, Toshiyuki Shibata, Hideo Miyake, Tetsushi Mori, Yutaka Tamaru, Mitsuyoshi Ueda, Peter Bossier

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    In this study, we identified and enumerated alginate-degrading bacteria in the gut of abalone over 1-year period. From a total of 360 colonies growing on agar medium enriched with alginate, 251 isolates (70%) had the ability to degrade alginate. In addition, a high number of viable alginate-degrading bacteria were detected throughout the survey period. Alginate-degrading bacteria were more abundant in the cold season relative to the summer season (107 vs. 104 CFU g-1, respectively). Strong positive correlation was also observed between the number of alginate-degrading bacteria and feed intake (R = 0.854; P < 0.01). The identified alginate-degrading bacteria comprised of 35 species grouped into 11 genera including Algibacter, Formosa, Polarybacter, Tamlana, Tenacibaculum (CFB group), Roseobacter, Ruegeria, Silicibacter (α-proteobacteria), Agarivorans, Shewanella and Vibrio (γ-proteobacteria) respectively. More than 80% of the isolated alginate-degrading bacteria belonged to the genus Vibrio, showing high homology to Vibrio cyclotorophicus, Vibrio splendidus, Vibrio halioticoli and Vibrio neonatus. Based on the results, it was suggested that algal-polysaccharide (alginate) degrading bacteria (mainly Vibrio) commonly exist in the gut of abalone and may play an important role in the degradation and digestion of the host's feed.

    Original languageEnglish
    JournalAquaculture Research
    DOIs
    Publication statusAccepted/In press - 2015

    Fingerprint

    Haliotis gigantea
    alginate
    abalone
    alginates
    digestive system
    bacterium
    Vibrio
    bacteria
    Ruegeria
    Vibrio halioticoli
    Agarivorans
    Tenacibaculum
    Vibrio splendidus
    Roseobacter
    Shewanella
    Eubacteria
    cold season
    Proteobacteria
    homology
    polysaccharide

    Keywords

    • Abalone
    • Alginate
    • Gut
    • Microbiota

    ASJC Scopus subject areas

    • Aquatic Science

    Cite this

    Temporal fluctuation in the abundance of alginate-degrading bacteria in the gut of abalone Haliotis gigantea over 1 year. / Tanaka, Reiji; Shibata, Toshiyuki; Miyake, Hideo; Mori, Tetsushi; Tamaru, Yutaka; Ueda, Mitsuyoshi; Bossier, Peter.

    In: Aquaculture Research, 2015.

    Research output: Contribution to journalArticle

    Tanaka, Reiji ; Shibata, Toshiyuki ; Miyake, Hideo ; Mori, Tetsushi ; Tamaru, Yutaka ; Ueda, Mitsuyoshi ; Bossier, Peter. / Temporal fluctuation in the abundance of alginate-degrading bacteria in the gut of abalone Haliotis gigantea over 1 year. In: Aquaculture Research. 2015.
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