Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum

Yusuke Seto, Tomoya Nakatani, Norihisa Masuyama, Shinichiro Taya, Minoru Kumai, Yasuko Minaki, Akiko Hamaguchi, Yukiko U. Inoue, Takayoshi Inoue, Satoshi Miyashita, Tomoyuki Fujiyama, Mayumi Yamada, Heather Chapman, Kenneth Campbell, Mark A. Magnuson, Christopher V. Wright, Yoshiya Kawaguchi, Kazuhiro Ikenaka, Hirohide Takebayashi, Shin'Ichi IshiwataYuichi Ono, Mikio Hoshino

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    37 Citations (Scopus)

    Abstract

    In the cerebellum, all GABAergic neurons are generated from the Ptf1a-expressing ventricular zone (Ptf1a domain). However, the machinery to produce different types of GABAergic neurons remains elusive. Here we show temporal regulation of distinct GABAergic neuron progenitors in the cerebellum. Within the Ptf1a domain at early stages, we find two subpopulations; dorsally and ventrally located progenitors that express Olig2 and Gsx1, respectively. Lineage tracing reveals the former are exclusively Purkinje cell progenitors (PCPs) and the latter Pax2-positive interneuron progenitors (PIPs). As development proceeds, PCPs gradually become PIPs starting from ventral to dorsal. In gain- and loss-of-function mutants for Gsx1 and Olig1/2, we observe abnormal transitioning from PCPs to PIPs at inappropriate developmental stages. Our findings suggest that the temporal identity transition of cerebellar GABAergic neuron progenitors from PCPs to PIPs is negatively regulated by Olig2 and positively by Gsx1, and contributes to understanding temporal control of neuronal progenitor identities.

    Original languageEnglish
    Article number4337
    JournalNature Communications
    Volume5
    DOIs
    Publication statusPublished - 2014 Feb 18

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Chemistry(all)
    • Physics and Astronomy(all)

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  • Cite this

    Seto, Y., Nakatani, T., Masuyama, N., Taya, S., Kumai, M., Minaki, Y., Hamaguchi, A., Inoue, Y. U., Inoue, T., Miyashita, S., Fujiyama, T., Yamada, M., Chapman, H., Campbell, K., Magnuson, M. A., Wright, C. V., Kawaguchi, Y., Ikenaka, K., Takebayashi, H., ... Hoshino, M. (2014). Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum. Nature Communications, 5, [4337]. https://doi.org/10.1038/ncomms4337