Temporal phase relation of circadian neural oscillations alters rfamide-related peptide-3 and testicular function in the mouse

Sumit Sethi, Kazuyoshi Tsutsui, Chandra Mohini Chaturvedi

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    In order to study the effect of the temporal synergism of neural oscillations on reproductive regulation and the response of RFamide-related peptide-3 (RFRP-3; a mammalian ortholog of avian gonadotropin-inhibitory hormone), expression of immunoreactive RFRP-3 in the neurons of the dorsomedial nucleus of the hypothalamus was monitored in sexually immature and mature laboratory mice (study I). In study II, the effects of serotonin and dopamine precursors (5-hydroxytryptophan and L-dihydroxyphenylalanine; injected daily, 8 or 12 h apart, for 13 days in 3-week-old mice) on testicular activity and immunoreactive RFRP-3 neurons were studied until 24 days after treatment. Results indicate high levels of expression of immunoreactive RFRP-3 in the sexually immature and 8-hour mice (simulating gonadal suppression), while a low level was noted in mature and 12-hour mice (simulating gonadal stimulation). These findings not only suggest the modulation of gonadal development in mice (during the course of puberty attainment) by changing the temporal phase relation of serotonergic and dopaminergic oscillations (as in some seasonally breeding species), but also demonstrate an inverse correlation of RFRP-3 neurons and gonadal activity in both control and experimental conditions.

    Original languageEnglish
    Pages (from-to)189-199
    Number of pages11
    JournalNeuroendocrinology
    Volume91
    Issue number2
    DOIs
    Publication statusPublished - 2010 Mar

    Fingerprint

    Neurons
    Mediodorsal Thalamic Nucleus
    5-Hydroxytryptophan
    Dihydroxyphenylalanine
    Puberty
    Gonadotropins
    Hypothalamus
    Breeding
    Dopamine
    Serotonin
    RFamide peptide
    Hormones
    Therapeutics

    Keywords

    • 5-Hydroxytryptophan
    • Gonadotropin- inhibitory hormone
    • L-DOPA
    • Mice
    • Reproduction
    • RFamide-related peptide-3
    • Testosterone

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism
    • Endocrine and Autonomic Systems
    • Cellular and Molecular Neuroscience

    Cite this

    Temporal phase relation of circadian neural oscillations alters rfamide-related peptide-3 and testicular function in the mouse. / Sethi, Sumit; Tsutsui, Kazuyoshi; Chaturvedi, Chandra Mohini.

    In: Neuroendocrinology, Vol. 91, No. 2, 03.2010, p. 189-199.

    Research output: Contribution to journalArticle

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