Tenascin-C is required for proliferation of astrocytes in primary culture

Hiroko Ikeshima-Kataoka*, Saburo Saito, Shigeki Yuasa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Astrocytes in primary culture can be classified morphologically into two types: fibrous astrocytes and protoplasmic astrocytes. To examine the role of tenascin-C (TN) in an in vitro astrocyte culture, primary cultures of astrocytes prepared from the brains of wild-type and of TN-deficient embryonic mice were analyzed. In primary culture of astrocytes from TN-deficient mice fibrous astrocytes did not appear and astrocytes did not become tile-shaped when they came in contact with each other. The rate of 5-bromo-2′-deoxyuridine incorporation in a cell proliferation assay was much lower for astrocytes from TN-deficient mice than for astrocytes from wild-type mice. These results suggest that TN is an essential molecule for maintaining the proliferation and proper morphology of astrocytes in primary culture.

Original languageEnglish
Pages (from-to)629-634
Number of pages6
JournalIn Vivo
Issue number4
Publication statusPublished - 2007 Jul
Externally publishedYes


  • 5-bromo-2′-deoxyufidine (BrdU)
  • Astrocytes
  • Cell proliferation
  • Glial fibrillary acidic protein (GFAP)
  • Tenascin-C (TN)

ASJC Scopus subject areas

  • Medicine(all)


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