Abstract
Astrocytes in primary culture can be classified morphologically into two types: fibrous astrocytes and protoplasmic astrocytes. To examine the role of tenascin-C (TN) in an in vitro astrocyte culture, primary cultures of astrocytes prepared from the brains of wild-type and of TN-deficient embryonic mice were analyzed. In primary culture of astrocytes from TN-deficient mice fibrous astrocytes did not appear and astrocytes did not become tile-shaped when they came in contact with each other. The rate of 5-bromo-2′-deoxyuridine incorporation in a cell proliferation assay was much lower for astrocytes from TN-deficient mice than for astrocytes from wild-type mice. These results suggest that TN is an essential molecule for maintaining the proliferation and proper morphology of astrocytes in primary culture.
| Original language | English |
|---|---|
| Pages (from-to) | 629-634 |
| Number of pages | 6 |
| Journal | In Vivo |
| Volume | 21 |
| Issue number | 4 |
| Publication status | Published - 2007 Jul |
| Externally published | Yes |
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Keywords
- 5-bromo-2′-deoxyufidine (BrdU)
- Astrocytes
- Cell proliferation
- Glial fibrillary acidic protein (GFAP)
- Tenascin-C (TN)
ASJC Scopus subject areas
- Medicine(all)
Cite this
Tenascin-C is required for proliferation of astrocytes in primary culture. / Ikeshima-Kataoka, Hiroko; Saito, Saburo; Yuasa, Shigeki.
In: In Vivo, Vol. 21, No. 4, 07.2007, p. 629-634.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tenascin-C is required for proliferation of astrocytes in primary culture
AU - Ikeshima-Kataoka, Hiroko
AU - Saito, Saburo
AU - Yuasa, Shigeki
PY - 2007/7
Y1 - 2007/7
N2 - Astrocytes in primary culture can be classified morphologically into two types: fibrous astrocytes and protoplasmic astrocytes. To examine the role of tenascin-C (TN) in an in vitro astrocyte culture, primary cultures of astrocytes prepared from the brains of wild-type and of TN-deficient embryonic mice were analyzed. In primary culture of astrocytes from TN-deficient mice fibrous astrocytes did not appear and astrocytes did not become tile-shaped when they came in contact with each other. The rate of 5-bromo-2′-deoxyuridine incorporation in a cell proliferation assay was much lower for astrocytes from TN-deficient mice than for astrocytes from wild-type mice. These results suggest that TN is an essential molecule for maintaining the proliferation and proper morphology of astrocytes in primary culture.
AB - Astrocytes in primary culture can be classified morphologically into two types: fibrous astrocytes and protoplasmic astrocytes. To examine the role of tenascin-C (TN) in an in vitro astrocyte culture, primary cultures of astrocytes prepared from the brains of wild-type and of TN-deficient embryonic mice were analyzed. In primary culture of astrocytes from TN-deficient mice fibrous astrocytes did not appear and astrocytes did not become tile-shaped when they came in contact with each other. The rate of 5-bromo-2′-deoxyuridine incorporation in a cell proliferation assay was much lower for astrocytes from TN-deficient mice than for astrocytes from wild-type mice. These results suggest that TN is an essential molecule for maintaining the proliferation and proper morphology of astrocytes in primary culture.
KW - 5-bromo-2′-deoxyufidine (BrdU)
KW - Astrocytes
KW - Cell proliferation
KW - Glial fibrillary acidic protein (GFAP)
KW - Tenascin-C (TN)
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UR - http://www.scopus.com/inward/citedby.url?scp=34547755544&partnerID=8YFLogxK
M3 - Article
C2 - 17708357
AN - SCOPUS:34547755544
VL - 21
SP - 629
EP - 634
JO - In Vivo
JF - In Vivo
SN - 0258-851X
IS - 4
ER -